Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120398..120923 Replicon plasmid pLH94-8
Accession NZ_CP035204
Organism Klebsiella pneumoniae strain LH94

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag EQH49_RS27905 Protein ID WP_025714517.1
Coordinates 120398..120703 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag EQH49_RS27910 Protein ID WP_001568025.1
Coordinates 120705..120923 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH49_RS27875 116109..116735 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
EQH49_RS28985 116732..117034 + 303 WP_071571079.1 hypothetical protein -
EQH49_RS27885 117474..118268 - 795 WP_004197635.1 site-specific integrase -
EQH49_RS27890 118466..119482 - 1017 WP_017899884.1 hypothetical protein -
EQH49_RS27895 119493..119807 - 315 WP_053389906.1 hypothetical protein -
EQH49_RS27900 119834..120229 - 396 WP_017899885.1 hypothetical protein -
EQH49_RS27905 120398..120703 - 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
EQH49_RS27910 120705..120923 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EQH49_RS27920 121491..122072 + 582 WP_032445670.1 hypothetical protein -
EQH49_RS27925 122233..123261 + 1029 WP_032445668.1 Abi family protein -
EQH49_RS27930 123555..124235 + 681 WP_032445676.1 hypothetical protein -
EQH49_RS27935 124373..125341 - 969 WP_023279773.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / mcr-8 / blaTEM-1B - 1..141746 141746
- flank IS/Tn mcr-8 - 124373..128225 3852


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T117369 WP_025714517.1 NZ_CP035204:c120703-120398 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T117369 NZ_CP035204:c120703-120398 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT117369 WP_001568025.1 NZ_CP035204:c120923-120705 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT117369 NZ_CP035204:c120923-120705 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References