Detailed information of TA system TA117366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 83828..84738
• Replicon: plasmid pLH94-1
• Accession: NZ_CP035203
• Organism: Klebsiella pneumoniae strain LH94

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EQH49_RS26395
• Protein ID: WP_032413375.1
• Coordinates: 84268..84738 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: EQH49_RS26390
• Protein ID: WP_032413443.1
• Coordinates: 83828..84271 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH49_RS26355	79662..80660	+	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-
EQH49_RS26360	80707..81885	+	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
EQH49_RS26365	82022..82369	+	348	WP_032413377.1	hypothetical protein	-
EQH49_RS26370	82427..82552	+	126	Protein_68	transposase	-
EQH49_RS26375	82686..82910	-	225	Protein_69	transposase	-
EQH49_RS26380	82930..83115	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
EQH49_RS26385	83216..83728	+	513	Protein_71	DUF4113 domain-containing protein	-
EQH49_RS26390	83828..84271	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
EQH49_RS26395	84268..84738	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
EQH49_RS26400	84848..85108	-	261	WP_017901409.1	hypothetical protein	-
EQH49_RS26405	85680..86057	-	378	Protein_75	transposase	-
EQH49_RS26415	86191..87159	+	969	WP_167829922.1	IS5 family transposase	-
EQH49_RS26420	87394..89127	-	1734	WP_004118832.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..202829	202829
	-	flank	IS/Tn	-	-	86191..87159	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T117366 WP_032413375.1 NZ_CP035203:84268-84738 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T117366 NZ_CP035203:84268-84738 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATAGCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT117366 WP_032413443.1 NZ_CP035203:83828-84271 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT117366 NZ_CP035203:83828-84271 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4