Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2541855..2542474 Replicon chromosome
Accession NZ_CP035202
Organism Klebsiella pneumoniae strain LH94

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EQH49_RS12440 Protein ID WP_002892050.1
Coordinates 2541855..2542073 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EQH49_RS12445 Protein ID WP_002892066.1
Coordinates 2542100..2542474 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH49_RS12410 2537898..2538161 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
EQH49_RS12415 2538161..2538301 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EQH49_RS12420 2538298..2538996 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
EQH49_RS12425 2539097..2540548 + 1452 WP_064145206.1 PLP-dependent aminotransferase family protein -
EQH49_RS12430 2540523..2540993 - 471 WP_002892026.1 YlaC family protein -
EQH49_RS28640 2541014..2541154 + 141 WP_004147370.1 hypothetical protein -
EQH49_RS12435 2541126..2541692 - 567 WP_002892030.1 maltose O-acetyltransferase -
EQH49_RS12440 2541855..2542073 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EQH49_RS12445 2542100..2542474 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EQH49_RS12450 2542960..2546106 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EQH49_RS12455 2546129..2547322 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117356 WP_002892050.1 NZ_CP035202:c2542073-2541855 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T117356 NZ_CP035202:c2542073-2541855 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT117356 WP_002892066.1 NZ_CP035202:c2542474-2542100 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT117356 NZ_CP035202:c2542474-2542100 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References