Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 1868842..1869533 Replicon chromosome
Accession NZ_CP035202
Organism Klebsiella pneumoniae strain LH94

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag EQH49_RS09185 Protein ID WP_025987721.1
Coordinates 1869192..1869533 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag EQH49_RS09180 Protein ID WP_019725272.1
Coordinates 1868842..1869168 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH49_RS09150 1864369..1865457 + 1089 WP_019725277.1 hypothetical protein -
EQH49_RS09155 1865540..1866430 + 891 WP_019725276.1 50S ribosome-binding GTPase -
EQH49_RS09165 1866995..1867684 + 690 WP_009309812.1 hypothetical protein -
EQH49_RS09170 1867896..1868342 + 447 WP_025987722.1 antirestriction protein -
EQH49_RS09175 1868352..1868828 + 477 WP_019725273.1 DNA repair protein RadC -
EQH49_RS09180 1868842..1869168 + 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EQH49_RS09185 1869192..1869533 + 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
EQH49_RS09190 1869648..1870481 + 834 WP_025987720.1 DUF4942 domain-containing protein -
EQH49_RS09195 1870568..1870879 + 312 WP_073568733.1 hypothetical protein -
EQH49_RS09200 1871304..1872539 + 1236 WP_124044824.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1849174..1887866 38692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T117354 WP_025987721.1 NZ_CP035202:1869192-1869533 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T117354 NZ_CP035202:1869192-1869533 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT117354 WP_019725272.1 NZ_CP035202:1868842-1869168 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT117354 NZ_CP035202:1868842-1869168 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References