Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 45818..46343 Replicon plasmid pLH375_1
Accession NZ_CP035197
Organism Klebsiella pneumoniae strain LH375

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag EP126_RS25690 Protein ID WP_006788214.1
Coordinates 46038..46343 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag EP126_RS25685 Protein ID WP_006788213.1
Coordinates 45818..46036 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP126_RS25655 41392..41823 + 432 WP_023302802.1 silver-binding protein SilE -
EP126_RS25660 41967..42317 + 351 WP_004213569.1 DUF305 domain-containing protein -
EP126_RS25665 42705..43613 - 909 WP_001372261.1 HNH endonuclease -
EP126_RS25670 44250..45224 + 975 WP_004213565.1 sensor domain-containing diguanylate cyclase -
EP126_RS25675 45403..45648 + 246 Protein_43 hypothetical protein -
EP126_RS25685 45818..46036 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EP126_RS25690 46038..46343 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
EP126_RS25695 46537..47553 + 1017 WP_006788215.1 hypothetical protein -
EP126_RS25700 47751..48545 + 795 WP_065801584.1 site-specific integrase -
EP126_RS25705 48985..49164 - 180 WP_006788217.1 hypothetical protein -
EP126_RS25710 49284..49910 - 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..52349 52349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T117346 WP_006788214.1 NZ_CP035197:46038-46343 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T117346 NZ_CP035197:46038-46343 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAGCTCTATCCGGTGG
TGCACATCGGGGACGAGAGCTGGCGCCTGATGACCACGGACATGGCCAGCGTCCCGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCCGCCACCGGGAAAATGACATCAAAAACGCAATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT117346 WP_006788213.1 NZ_CP035197:45818-46036 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT117346 NZ_CP035197:45818-46036 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATCGACAGCGACAGCTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAAGCCCGACGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References