Detailed information of TA system TA117345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 18938..19581
• Replicon: plasmid pLH375_1
• Accession: NZ_CP035197
• Organism: Klebsiella pneumoniae strain LH375

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EP126_RS25535
• Protein ID: WP_152660214.1
• Coordinates: 18938..19354 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: EP126_RS25540
• Protein ID: WP_001261276.1
• Coordinates: 19351..19581 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP126_RS25520	14443..15462	+	1020	WP_152660213.1	type 1 fimbrial protein	-
EP126_RS25525	15659..17182	-	1524	WP_117282697.1	EAL domain-containing protein	-
EP126_RS25530	17617..18843	-	1227	Protein_16	IS3 family transposase	-
EP126_RS25535	18938..19354	-	417	WP_152660214.1	type II toxin-antitoxin system VapC family toxin	Toxin
EP126_RS25540	19351..19581	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EP126_RS25550	19899..20078	+	180	WP_004118349.1	type II toxin-antitoxin system ParD family antitoxin	-
EP126_RS25555	20065..20163	+	99	WP_004118688.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EP126_RS25565	20542..20976	-	435	WP_009309894.1	hypothetical protein	-
EP126_RS25570	21192..22592	-	1401	WP_004152084.1	copper resistance membrane spanning protein PcoS	-
EP126_RS25575	22589..23269	-	681	WP_001188930.1	copper response regulator transcription factor PcoR	-
EP126_RS25580	23324..24253	-	930	WP_004118344.1	copper resistance inner membrane protein PcoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52349	52349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T117345 WP_152660214.1 NZ_CP035197:c19354-18938 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWIK

Nucleotide

• Length: 417 bp

>T117345 NZ_CP035197:c19354-18938 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGATACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACATATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACAGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTGGTTCTGG
AAGACTGGATTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT117345 WP_001261276.1 NZ_CP035197:c19581-19351 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT117345 NZ_CP035197:c19581-19351 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGTTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023