Detailed information of TA system TA117343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 5046857..5047635
• Replicon: chromosome
• Accession: NZ_CP035196
• Organism: Klebsiella pneumoniae strain LH375

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EP126_RS24785
• Protein ID: WP_031591140.1
• Coordinates: 5047147..5047635 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: EP126_RS24780
• Protein ID: WP_004150912.1
• Coordinates: 5046857..5047150 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP126_RS24760	5042065..5042667	-	603	WP_004174410.1	short chain dehydrogenase	-
EP126_RS24765	5042765..5043676	+	912	WP_004181308.1	LysR family transcriptional regulator	-
EP126_RS24770	5043677..5044825	-	1149	WP_004174416.1	PLP-dependent aspartate aminotransferase family protein	-
EP126_RS24775	5044836..5046212	-	1377	WP_004217775.1	cystathionine beta-synthase	-
EP126_RS24780	5046857..5047150	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
EP126_RS24785	5047147..5047635	+	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
EP126_RS24790	5047716..5051459	-	3744	Protein_4792	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5046608..5071417	24809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T117343 WP_031591140.1 NZ_CP035196:5047147-5047635 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T117343 NZ_CP035196:5047147-5047635 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTGTGTTGCGGCAGCGATTCTAACGTAC
TGGCGTATTACTCGCTCGCGTCCAGTGCGGTAATGACTAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTAGTAGTGCTGGGGCGTCTGGCGGTGGATCAGTCTCTACATGGACGGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGGGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTATCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT117343 WP_004150912.1 NZ_CP035196:5046857-5047150 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT117343 NZ_CP035196:5046857-5047150 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4