Detailed information of TA system TA117340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4522997..4523736
• Replicon: chromosome
• Accession: NZ_CP035196
• Organism: Klebsiella pneumoniae strain LH375

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: EP126_RS22145
• Protein ID: WP_021312536.1
• Coordinates: 4523251..4523736 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: EP126_RS22140
• Protein ID: WP_003026799.1
• Coordinates: 4522997..4523263 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP126_RS22125	4519641..4521010	+	1370	WP_087661561.1	IS3 family transposase	-
EP126_RS22130	4521167..4522287	+	1121	WP_088765928.1	IS3 family transposase	-
EP126_RS22135	4522436..4522864	+	429	WP_004901287.1	GFA family protein	-
EP126_RS22140	4522997..4523263	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
EP126_RS22145	4523251..4523736	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
EP126_RS22150	4524080..4524232	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
EP126_RS22155	4524534..4526153	+	1620	WP_004901278.1	ATP-binding cassette domain-containing protein	-
EP126_RS22160	4526252..4526464	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
EP126_RS22165	4526716..4527006	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
EP126_RS22170	4527252..4528607	-	1356	WP_004901270.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T117340 WP_021312536.1 NZ_CP035196:4523251-4523736 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T117340 NZ_CP035196:4523251-4523736 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT117340 WP_003026799.1 NZ_CP035196:4522997-4523263 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT117340 NZ_CP035196:4522997-4523263 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66