Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4240668..4241293 Replicon chromosome
Accession NZ_CP035196
Organism Klebsiella pneumoniae strain LH375

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EP126_RS20765 Protein ID WP_002882817.1
Coordinates 4240668..4241051 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EP126_RS20770 Protein ID WP_004150355.1
Coordinates 4241051..4241293 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP126_RS20750 4238034..4238936 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EP126_RS20755 4238933..4239568 + 636 WP_064147423.1 formate dehydrogenase cytochrome b556 subunit -
EP126_RS20760 4239565..4240494 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EP126_RS20765 4240668..4241051 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EP126_RS20770 4241051..4241293 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EP126_RS20775 4241498..4242415 + 918 WP_064147424.1 alpha/beta hydrolase -
EP126_RS20780 4242429..4243370 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EP126_RS20785 4243415..4243852 - 438 WP_064147425.1 D-tyrosyl-tRNA(Tyr) deacylase -
EP126_RS20790 4243849..4244709 - 861 WP_004146232.1 virulence factor BrkB family protein -
EP126_RS20795 4244703..4245302 - 600 WP_152660152.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T117339 WP_002882817.1 NZ_CP035196:c4241051-4240668 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T117339 NZ_CP035196:c4241051-4240668 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT117339 WP_004150355.1 NZ_CP035196:c4241293-4241051 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT117339 NZ_CP035196:c4241293-4241051 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References