Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3044072..3044691 Replicon chromosome
Accession NZ_CP035196
Organism Klebsiella pneumoniae strain LH375

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EP126_RS14950 Protein ID WP_002892050.1
Coordinates 3044473..3044691 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EP126_RS14945 Protein ID WP_002892066.1
Coordinates 3044072..3044446 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP126_RS14935 3039224..3040417 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EP126_RS14940 3040440..3043586 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EP126_RS14945 3044072..3044446 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EP126_RS14950 3044473..3044691 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EP126_RS14955 3044850..3045416 + 567 WP_002892030.1 maltose O-acetyltransferase -
EP126_RS26875 3045388..3045528 - 141 WP_004147370.1 hypothetical protein -
EP126_RS14960 3045549..3046019 + 471 WP_002892026.1 YlaC family protein -
EP126_RS14965 3045994..3047445 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
EP126_RS14970 3047546..3048244 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
EP126_RS14975 3048241..3048381 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EP126_RS14980 3048381..3048644 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117336 WP_002892050.1 NZ_CP035196:3044473-3044691 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T117336 NZ_CP035196:3044473-3044691 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT117336 WP_002892066.1 NZ_CP035196:3044072-3044446 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT117336 NZ_CP035196:3044072-3044446 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References