Detailed information of TA system TA117325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 3402928..3403738
• Replicon: chromosome
• Accession: NZ_CP035194
• Organism: Klebsiella pneumoniae strain LH102-A

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A483R2P9
• Locus tag: EPO92_RS16835
• Protein ID: WP_048977643.1
• Coordinates: 3403205..3403738 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: EPO92_RS16830
• Protein ID: WP_117075885.1
• Coordinates: 3402928..3403194 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPO92_RS16810	3398800..3399144	-	345	WP_152659840.1	cation efflux system protein CusF	-
EPO92_RS16815	3399162..3400547	-	1386	WP_085803494.1	efflux transporter outer membrane subunit	-
EPO92_RS16820	3400719..3401402	+	684	WP_002887273.1	copper response regulator transcription factor CusR	-
EPO92_RS16825	3401392..3402825	+	1434	WP_087759796.1	Cu(+)/Ag(+) sensor histidine kinase	-
EPO92_RS16830	3402928..3403194	+	267	WP_117075885.1	DUF1778 domain-containing protein	Antitoxin
EPO92_RS16835	3403205..3403738	+	534	WP_048977643.1	GNAT family N-acetyltransferase	Toxin
EPO92_RS16840	3403786..3404907	-	1122	WP_002887282.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19842.69 Da
• Isoelectric Point: 5.2614

>T117325 WP_048977643.1 NZ_CP035194:3403205-3403738 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGYDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLLSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T117325 NZ_CP035194:3403205-3403738 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATATGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCTGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10018.51 Da
• Isoelectric Point: 10.5848

>AT117325 WP_117075885.1 NZ_CP035194:3402928-3403194 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDHKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT117325 NZ_CP035194:3402928-3403194 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATCATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A483R2P9