Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2816880..2817505 Replicon chromosome
Accession NZ_CP035194
Organism Klebsiella pneumoniae strain LH102-A

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EPO92_RS14060 Protein ID WP_002882817.1
Coordinates 2817122..2817505 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EPO92_RS14055 Protein ID WP_004150355.1
Coordinates 2816880..2817122 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPO92_RS14030 2812871..2813470 + 600 WP_004151865.1 glucose-1-phosphatase -
EPO92_RS14035 2813464..2814324 + 861 WP_004146232.1 virulence factor BrkB family protein -
EPO92_RS14040 2814321..2814758 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EPO92_RS14045 2814803..2815744 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EPO92_RS14050 2815758..2816675 - 918 WP_009309725.1 alpha/beta hydrolase -
EPO92_RS14055 2816880..2817122 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EPO92_RS14060 2817122..2817505 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EPO92_RS14065 2817679..2818608 - 930 WP_101985522.1 formate dehydrogenase accessory protein FdhE -
EPO92_RS14070 2818605..2819240 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EPO92_RS14075 2819237..2820139 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T117322 WP_002882817.1 NZ_CP035194:2817122-2817505 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T117322 NZ_CP035194:2817122-2817505 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT117322 WP_004150355.1 NZ_CP035194:2816880-2817122 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT117322 NZ_CP035194:2816880-2817122 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References