Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509876..510512 Replicon chromosome
Accession NZ_CP035165
Organism Bacillus subtilis strain SRCM103881

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQI56_RS02610 Protein ID WP_003156187.1
Coordinates 510162..510512 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQI56_RS02605 Protein ID WP_003225183.1
Coordinates 509876..510157 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQI56_RS02585 506236..506835 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
EQI56_RS02590 506930..507295 + 366 WP_015252768.1 holo-ACP synthase -
EQI56_RS02595 507461..508477 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
EQI56_RS02600 508591..509760 + 1170 WP_015252766.1 alanine racemase -
EQI56_RS02605 509876..510157 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQI56_RS02610 510162..510512 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQI56_RS02615 510627..511451 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
EQI56_RS02620 511456..511821 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQI56_RS02625 511825..512226 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
EQI56_RS02630 512238..513245 + 1008 WP_128472209.1 phosphoserine phosphatase RsbU -
EQI56_RS02635 513307..513636 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
EQI56_RS02640 513633..514115 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EQI56_RS02645 514081..514869 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
EQI56_RS02650 514869..515468 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117266 WP_003156187.1 NZ_CP035165:510162-510512 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117266 NZ_CP035165:510162-510512 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117266 WP_003225183.1 NZ_CP035165:509876-510157 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117266 NZ_CP035165:509876-510157 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References