Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516645..517281 Replicon chromosome
Accession NZ_CP035164
Organism Bacillus subtilis strain SRCM104005

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQI48_RS02650 Protein ID WP_003156187.1
Coordinates 516931..517281 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQI48_RS02645 Protein ID WP_003225183.1
Coordinates 516645..516926 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQI48_RS02625 513005..513604 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
EQI48_RS02630 513699..514064 + 366 WP_015252768.1 holo-ACP synthase -
EQI48_RS02635 514230..515246 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
EQI48_RS02640 515360..516529 + 1170 WP_015252766.1 alanine racemase -
EQI48_RS02645 516645..516926 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQI48_RS02650 516931..517281 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQI48_RS02655 517396..518220 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
EQI48_RS02660 518225..518590 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQI48_RS02665 518594..518995 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
EQI48_RS02670 519007..520014 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
EQI48_RS02675 520076..520405 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
EQI48_RS02680 520402..520884 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EQI48_RS02685 520850..521638 + 789 WP_029727187.1 RNA polymerase sigma factor SigB -
EQI48_RS02690 521638..522237 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117264 WP_003156187.1 NZ_CP035164:516931-517281 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117264 NZ_CP035164:516931-517281 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117264 WP_003225183.1 NZ_CP035164:516645-516926 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117264 NZ_CP035164:516645-516926 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References