Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 545518..546154 Replicon chromosome
Accession NZ_CP035162
Organism Bacillus subtilis strain SRCM103886

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EQH95_RS02710 Protein ID WP_003156187.1
Coordinates 545804..546154 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EQH95_RS02705 Protein ID WP_003225183.1
Coordinates 545518..545799 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH95_RS02685 541877..542476 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
EQH95_RS02690 542571..542936 + 366 WP_014478897.1 holo-ACP synthase -
EQH95_RS02695 543102..544118 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
EQH95_RS02700 544233..545402 + 1170 WP_014478898.1 alanine racemase -
EQH95_RS02705 545518..545799 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EQH95_RS02710 545804..546154 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EQH95_RS02715 546270..547094 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
EQH95_RS02720 547099..547464 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EQH95_RS02725 547468..547869 + 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
EQH95_RS02730 547881..548888 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
EQH95_RS02735 548957..549286 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
EQH95_RS02740 549283..549765 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EQH95_RS02745 549731..550519 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
EQH95_RS02750 550519..551118 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117256 WP_003156187.1 NZ_CP035162:545804-546154 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117256 NZ_CP035162:545804-546154 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117256 WP_003225183.1 NZ_CP035162:545518-545799 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117256 NZ_CP035162:545518-545799 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References