Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 62132..62733 Replicon plasmid unnamed1
Accession NZ_CP035137
Organism Enterococcus faecium strain SRCM103341

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V7YM55
Locus tag EQI29_RS13530 Protein ID WP_002330364.1
Coordinates 62132..62476 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B3Q8V0
Locus tag EQI29_RS13535 Protein ID WP_002318881.1
Coordinates 62470..62733 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQI29_RS13490 57491..57802 - 312 WP_104886006.1 hypothetical protein -
EQI29_RS13495 58696..59301 - 606 Protein_62 recombinase family protein -
EQI29_RS13500 59592..59789 + 198 WP_002291096.1 hypothetical protein -
EQI29_RS13505 59882..60046 + 165 WP_128440566.1 class II bacteriocin -
EQI29_RS13510 60051..60317 + 267 WP_002291092.1 hypothetical protein -
EQI29_RS13515 60691..60900 + 210 Protein_66 recombinase family protein -
EQI29_RS13525 61712..62017 - 306 Protein_68 IS982 family transposase -
EQI29_RS13530 62132..62476 - 345 WP_002330364.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EQI29_RS13535 62470..62733 - 264 WP_002318881.1 PbsX family transcriptional regulator Antitoxin
EQI29_RS13540 62920..63459 + 540 Protein_71 IS30 family transposase -
EQI29_RS13545 63567..64736 - 1170 WP_047936661.1 IS256 family transposase -
EQI29_RS13550 64898..65986 + 1089 WP_128440547.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..211011 211011
- inside IScluster/Tn - - 60955..64736 3781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13024.11 Da        Isoelectric Point: 8.8971

>T117252 WP_002330364.1 NZ_CP035137:c62476-62132 [Enterococcus faecium]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Download         Length: 345 bp

>T117252 NZ_CP035137:c62476-62132 [Enterococcus faecium]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10071.69 Da        Isoelectric Point: 5.2300

>AT117252 WP_002318881.1 NZ_CP035137:c62733-62470 [Enterococcus faecium]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Download         Length: 264 bp

>AT117252 NZ_CP035137:c62733-62470 [Enterococcus faecium]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGTAGAGAACTATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7YM55

Antitoxin

Source ID Structure
AlphaFold DB A0A6B3Q8V0

References