Detailed information of TA system TA117246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3468568..3469184
• Replicon: chromosome
• Accession: NZ_CP035129
• Organism: Kosakonia cowanii strain FBS 223

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A807LE76
• Locus tag: EQG67_RS16445
• Protein ID: WP_023478287.1
• Coordinates: 3468966..3469184 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EQG67_RS16440
• Protein ID: WP_023478264.1
• Coordinates: 3468568..3468942 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQG67_RS16430	3463666..3464865	+	1200	WP_128483482.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
EQG67_RS16435	3464888..3468037	+	3150	WP_076769497.1	multidrug efflux RND transporter permease subunit AcrB	-
EQG67_RS16440	3468568..3468942	+	375	WP_023478264.1	Hha toxicity modulator TomB	Antitoxin
EQG67_RS16445	3468966..3469184	+	219	WP_023478287.1	hemolysin expression modulator Hha	Toxin
EQG67_RS16450	3469260..3469826	+	567	WP_128483483.1	maltose O-acetyltransferase	-
EQG67_RS16455	3469930..3470433	+	504	WP_128483484.1	YlaC family protein	-
EQG67_RS16460	3470560..3471261	+	702	WP_076769495.1	GNAT family N-acetyltransferase	-
EQG67_RS16465	3471258..3471398	-	141	WP_023478279.1	type B 50S ribosomal protein L36	-
EQG67_RS16470	3471402..3471662	-	261	WP_023478283.1	type B 50S ribosomal protein L31	-
EQG67_RS16475	3471924..3472952	+	1029	WP_128483485.1	isopenicillin N synthase family oxygenase	-
EQG67_RS16480	3472975..3473781	+	807	WP_128483486.1	MetQ/NlpA family ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8657.04 Da
• Isoelectric Point: 7.9892

>T117246 WP_023478287.1 NZ_CP035129:3468966-3469184 [Kosakonia cowanii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELTDDELTVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T117246 NZ_CP035129:3468966-3469184 [Kosakonia cowanii]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGGCGCTGCCAATCAATTGATACGCTCGAACGCGT
TATTGAAAAAAATAAATACGAACTGACCGACGATGAGCTTACGGTGTTTTATTCAGCGGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCGTCGGTGTGGAAATTTGTTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14420.22 Da
• Isoelectric Point: 6.0581

>AT117246 WP_023478264.1 NZ_CP035129:3468568-3468942 [Kosakonia cowanii]
MDEYSPKRHDIAQLKFLCESLHQECLANLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKHDKDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGGKLFRSFDNVSKANPVSHSC

Nucleotide

• Length: 375 bp

>AT117246 NZ_CP035129:3468568-3468942 [Kosakonia cowanii]
ATGGACGAATACTCGCCGAAAAGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAATCGTTGCACCAGGAGTGCCTCGC
CAATCTGGAAGAGAGCCATCATGGATGGGTTAACGATCCCACATCAGCCATCAACCTGCAGTTAAATGAGCTGATTGAGC
ATATTGCAACGTTCGCGCTGAATTATAAAATTAAGCACGACAAAGATAATAAACTGATTGAATTGATCGACGAATACCTT
GATGACACGTTTATGTTGTTCAGCAGTTACGGTATTAATACACACGACTTACAGAAATGGCGTAAAACAGGCGGTAAGCT
GTTTCGCAGTTTCGACAATGTAAGCAAGGCTAATCCAGTAAGTCACTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A807LE76