Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 159055..159580 Replicon plasmid pEC25-1
Accession NZ_CP035124
Organism Escherichia coli strain EC25

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag EQK40_RS24540 Protein ID WP_001159871.1
Coordinates 159055..159360 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag EQK40_RS24545 Protein ID WP_000813630.1
Coordinates 159362..159580 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQK40_RS24525 155017..156183 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EQK40_RS24530 156771..157526 - 756 WP_000852146.1 replication initiation protein RepE -
EQK40_RS24535 158248..159054 - 807 WP_000016970.1 site-specific integrase -
EQK40_RS24540 159055..159360 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
EQK40_RS24545 159362..159580 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EQK40_RS24555 160140..160370 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EQK40_RS24560 160367..160783 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
EQK40_RS24565 160858..162423 + 1566 WP_001128474.1 AAA family ATPase -
EQK40_RS24570 162408..163430 + 1023 WP_000361402.1 helicase UvrD -
EQK40_RS24575 163684..164381 - 698 Protein_206 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) / sitABCD / aadA5 / blaOXA-1 / blaTEM-1B / blaCTX-M-3 / qnrS1 iutA / iucD / iucC / iucB / iucA 1..227349 227349
- flank IS/Tn - - 163684..164187 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T117234 WP_001159871.1 NZ_CP035124:c159360-159055 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T117234 NZ_CP035124:c159360-159055 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT117234 WP_000813630.1 NZ_CP035124:c159580-159362 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT117234 NZ_CP035124:c159580-159362 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References