Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72738..73263 Replicon plasmid pEC25-1
Accession NZ_CP035124
Organism Escherichia coli strain EC25

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag EQK40_RS23870 Protein ID WP_013023785.1
Coordinates 72738..73043 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag EQK40_RS23875 Protein ID WP_001568025.1
Coordinates 73045..73263 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQK40_RS23840 68449..69075 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
EQK40_RS23845 69195..69374 + 180 WP_006788217.1 hypothetical protein -
EQK40_RS23850 69814..70608 - 795 WP_004197635.1 site-specific integrase -
EQK40_RS23855 70806..71822 - 1017 WP_017899884.1 hypothetical protein -
EQK40_RS23860 71833..72147 - 315 WP_053389906.1 hypothetical protein -
EQK40_RS23865 72174..72569 - 396 WP_017899885.1 hypothetical protein -
EQK40_RS23870 72738..73043 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
EQK40_RS23875 73045..73263 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EQK40_RS23895 74081..74371 - 291 WP_013023783.1 hypothetical protein -
EQK40_RS23900 74368..75495 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
EQK40_RS23905 75529..77121 - 1593 WP_015344964.1 hypothetical protein -
EQK40_RS23910 77328..78107 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) / sitABCD / aadA5 / blaOXA-1 / blaTEM-1B / blaCTX-M-3 / qnrS1 iutA / iucD / iucC / iucB / iucA 1..227349 227349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T117229 WP_013023785.1 NZ_CP035124:c73043-72738 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T117229 NZ_CP035124:c73043-72738 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT117229 WP_001568025.1 NZ_CP035124:c73263-73045 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT117229 NZ_CP035124:c73263-73045 [Escherichia coli]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References