Detailed information of TA system TA117215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1334145..1334770
• Replicon: chromosome
• Accession: NZ_CP035123
• Organism: Escherichia coli strain EC25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EQK40_RS06535
• Protein ID: WP_000911330.1
• Coordinates: 1334372..1334770 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EQK40_RS06530
• Protein ID: WP_000450524.1
• Coordinates: 1334145..1334372 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQK40_RS06505	1329948..1330418	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EQK40_RS06510	1330418..1330990	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EQK40_RS06515	1331136..1332014	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EQK40_RS06520	1332031..1333065	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EQK40_RS06525	1333278..1333991	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EQK40_RS06530	1334145..1334372	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EQK40_RS06535	1334372..1334770	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EQK40_RS06540	1334917..1335780	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
EQK40_RS06545	1335795..1337810	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
EQK40_RS06550	1337884..1338582	+	699	WP_000679823.1	esterase	-
EQK40_RS06555	1338692..1338892	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T117215 WP_000911330.1 NZ_CP035123:1334372-1334770 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T117215 NZ_CP035123:1334372-1334770 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT117215 WP_000450524.1 NZ_CP035123:1334145-1334372 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT117215 NZ_CP035123:1334145-1334372 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33