Detailed information of TA system TA117207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 178816..179345
• Replicon: chromosome
• Accession: NZ_CP035112
• Organism: Coxiella burnetii strain nine mile phase II

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: EP112_RS01375
• Protein ID: WP_005769718.1
• Coordinates: 179070..179345 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: EP112_RS01370
• Protein ID: WP_005769716.1
• Coordinates: 178816..179070 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP112_RS01330	173886..174260	+	375	WP_005769706.1	hypothetical protein	-
EP112_RS01335	174305..175003	+	699	WP_010958581.1	outer membrane beta-barrel protein	-
EP112_RS01340	175067..176008	+	942	WP_005769709.1	S49 family peptidase	-
EP112_RS01345	176021..176896	+	876	WP_010958582.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
EP112_RS01350	176872..176985	+	114	Protein_192	type II toxin-antitoxin system YoeB family toxin	-
EP112_RS01355	177045..177179	+	135	WP_010958583.1	hypothetical protein	-
EP112_RS01360	177340..178358	+	1019	Protein_194	IS110-like element IS1111A family transposase	-
EP112_RS01365	178309..178653	-	345	Protein_195	hypothetical protein	-
EP112_RS01370	178816..179070	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EP112_RS01375	179070..179345	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
EP112_RS01380	179318..179803	-	486	WP_005769725.1	dihydrofolate reductase	-
EP112_RS01385	179800..180603	-	804	WP_010958584.1	epoxyqueuosine reductase QueH	-
EP112_RS01390	180799..181086	+	288	WP_010958585.1	acylphosphatase	-
EP112_RS01395	181062..181616	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
EP112_RS01400	181629..182573	-	945	WP_010958586.1	methionyl-tRNA formyltransferase	-
EP112_RS01405	182761..183687	+	927	WP_010958587.1	DNA-processing protein DprA	-
EP112_RS01410	183684..184163	+	480	WP_010958588.1	DUF494 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	177340..178326	986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T117207 WP_005769718.1 NZ_CP035112:179070-179345 [Coxiella burnetii]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T117207 NZ_CP035112:179070-179345 [Coxiella burnetii]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT117207 WP_005769716.1 NZ_CP035112:178816-179070 [Coxiella burnetii]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT117207 NZ_CP035112:178816-179070 [Coxiella burnetii]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4