Detailed information of TA system TA117186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 967189..967718
• Replicon: chromosome
• Accession: NZ_CP035091
• Organism: Paracoccus denitrificans strain ATCC 19367

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A1B8Q3
• Locus tag: EO213_RS19500
• Protein ID: WP_011750065.1
• Coordinates: 967431..967718 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0V0Q4K1
• Locus tag: EO213_RS19495
• Protein ID: WP_010396097.1
• Coordinates: 967189..967434 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO213_RS19465	962444..962896	-	453	WP_011750059.1	CoA-binding protein	-
EO213_RS19470	963080..963493	-	414	WP_011750060.1	YHS domain-containing protein	-
EO213_RS19475	963594..964391	-	798	WP_011750061.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
EO213_RS19485	964873..965730	+	858	WP_011750063.1	hypothetical protein	-
EO213_RS19490	965723..967084	+	1362	WP_011750064.1	plasmid recombination protein	-
EO213_RS19495	967189..967434	+	246	WP_010396097.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EO213_RS19500	967431..967718	+	288	WP_011750065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EO213_RS19505	967777..968310	+	534	WP_041530416.1	hypothetical protein	-
EO213_RS19510	968535..969707	-	1173	WP_011750067.1	ATP-binding protein	-
EO213_RS19515	970035..970244	-	210	WP_011748680.1	hypothetical protein	-
EO213_RS19520	970386..970664	-	279	WP_041530417.1	hypothetical protein	-
EO213_RS19525	970661..971170	-	510	WP_011750069.1	hypothetical protein	-
EO213_RS19530	971167..971349	-	183	WP_028717528.1	hypothetical protein	-
EO213_RS19535	971346..971669	-	324	WP_011750070.1	zinc ribbon domain-containing protein	-
EO213_RS19540	971779..972024	+	246	WP_011750071.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	964620..983343	18723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-76)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10768.19 Da
• Isoelectric Point: 6.0765

>T117186 WP_011750065.1 NZ_CP035091:967431-967718 [Paracoccus denitrificans]
VKALAFTVAATVDLEDIWDYTEGEWGLAQARRYAEELRDTCHALASGERRGRKVDVRPGYLKCLSGSHIIFFRDLGDTLE
IVRILHGAQDVERHL

Nucleotide

• Length: 288 bp

>T117186 NZ_CP035091:967431-967718 [Paracoccus denitrificans]
GTGAAGGCATTGGCCTTCACCGTCGCGGCAACGGTCGATCTGGAGGATATCTGGGATTATACGGAAGGCGAATGGGGTCT
GGCGCAAGCCCGGCGGTATGCGGAGGAGCTGCGCGACACCTGCCACGCGCTGGCTTCGGGCGAAAGGCGCGGCAGGAAAG
TTGACGTTCGGCCCGGCTATCTGAAGTGCCTGAGCGGGTCGCATATCATCTTTTTCCGGGATCTGGGCGATACGCTGGAA
ATCGTCCGCATCCTGCACGGTGCACAGGACGTCGAGCGGCATTTGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8708.75 Da
• Isoelectric Point: 6.4631

>AT117186 WP_010396097.1 NZ_CP035091:967189-967434 [Paracoccus denitrificans]
MSRNTSVSLGDHFVSFIDAQVKGGRYGSASDVVRAGLRLLEEHEAKVKALQDALIAGEDSGRAEGFDLDQFIARKMAGRN
A

Nucleotide

• Length: 246 bp

>AT117186 NZ_CP035091:967189-967434 [Paracoccus denitrificans]
ATGTCCCGCAACACTTCGGTTTCGCTCGGCGATCACTTCGTCAGCTTCATCGACGCGCAGGTGAAGGGCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGGGCGGGGCTGCGGCTTCTGGAGGAGCACGAGGCCAAGGTAAAGGCCCTCCAGGACGCCC
TGATCGCGGGAGAGGACTCCGGCCGGGCGGAGGGCTTCGACCTCGATCAGTTCATTGCCCGCAAGATGGCCGGTCGCAAT
GCGTGA

Structures

Toxin

• AlphaFold DB: A1B8Q3

Antitoxin

• AlphaFold DB: A0A0V0Q4K1