Detailed information of TA system TA117184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 357770..358455
• Replicon: chromosome
• Accession: NZ_CP035091
• Organism: Paracoccus denitrificans strain ATCC 19367

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A1B6X9
• Locus tag: EO213_RS16240
• Protein ID: WP_011749459.1
• Coordinates: 357770..358195 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A1B6Y0
• Locus tag: EO213_RS16245
• Protein ID: WP_011749460.1
• Coordinates: 358192..358455 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO213_RS16225	354444..356453	+	2010	WP_011749455.1	conjugal transfer protein TraG	-
EO213_RS16230	356484..356879	+	396	Protein_353	CopG family transcriptional regulator	-
EO213_RS16235	357031..357573	-	543	WP_041530330.1	AAA family ATPase	-
EO213_RS16240	357770..358195	-	426	WP_011749459.1	type II toxin-antitoxin system VapC family toxin	Toxin
EO213_RS16245	358192..358455	-	264	WP_011749460.1	plasmid stabilization protein	Antitoxin
EO213_RS16250	358735..359739	+	1005	WP_011749461.1	P-type conjugative transfer ATPase TrbB	-
EO213_RS16255	359736..360068	+	333	WP_011749462.1	TrbC/VirB2 family protein	-
EO213_RS16260	360068..360349	+	282	WP_011749463.1	VirB3 family type IV secretion system protein	-
EO213_RS16265	360362..362791	+	2430	Protein_360	conjugal transfer protein TrbE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-Ih	-	180860..360853	179993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15061.32 Da
• Isoelectric Point: 4.7222

>T117184 WP_011749459.1 NZ_CP035091:c358195-357770 [Paracoccus denitrificans]
MILLDTNVVSEPMREAPNPGVIGWIDDQAIETLHLSAITVAEVRFGIAVLPDGKRKARLQSRFETEILPLFADRILPFDL
AASEAFAALMAHARDKSAAIGKADGYIAAIAAAHRLTVATRDTAPFLAAGVTVIDPWDEAG

Nucleotide

• Length: 426 bp

>T117184 NZ_CP035091:c358195-357770 [Paracoccus denitrificans]
ATGATCCTGCTTGACACGAATGTCGTCTCCGAACCGATGCGGGAGGCGCCGAATCCCGGCGTGATCGGCTGGATCGACGA
CCAGGCGATCGAGACGCTGCACCTCTCGGCCATCACCGTGGCGGAGGTCCGTTTCGGGATCGCAGTGCTTCCCGACGGCA
AGCGCAAGGCCCGCCTGCAATCCCGTTTCGAAACCGAGATCCTGCCGCTCTTCGCCGACCGCATCCTGCCCTTCGACCTC
GCGGCCTCGGAAGCCTTCGCGGCGCTGATGGCCCATGCCCGGGACAAGAGCGCGGCCATAGGCAAGGCGGACGGCTACAT
CGCCGCCATTGCCGCCGCCCATCGGCTGACCGTGGCCACGCGCGATACGGCACCGTTCCTGGCCGCCGGCGTCACGGTCA
TCGACCCGTGGGACGAGGCCGGTTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9852.22 Da
• Isoelectric Point: 10.6440

>AT117184 WP_011749460.1 NZ_CP035091:c358455-358192 [Paracoccus denitrificans]
MPAITIRNLSAETHRALKMRAAQHGRSTEAEVRDILEQAARPQHRVRLGSLLAEIGREARMTKEEHALFETAIRRDRAPA
QPIDFGE

Nucleotide

• Length: 264 bp

>AT117184 NZ_CP035091:c358455-358192 [Paracoccus denitrificans]
ATGCCCGCCATCACCATTCGCAATCTGTCCGCCGAGACGCATCGGGCGCTGAAGATGCGCGCGGCGCAGCATGGGCGCAG
CACCGAGGCCGAGGTGCGCGACATTCTCGAACAGGCAGCACGACCCCAGCACCGTGTGCGGCTGGGATCGCTGCTGGCCG
AGATCGGCCGTGAGGCACGGATGACCAAGGAAGAGCACGCCCTGTTCGAGACCGCGATCCGGCGTGACCGGGCCCCTGCC
CAACCGATCGATTTCGGCGAATGA

Structures

Toxin

• AlphaFold DB: A1B6X9

Antitoxin

• AlphaFold DB: A1B6Y0