Detailed information of TA system TA117140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2012851..2013441
• Replicon: chromosome
• Accession: NZ_CP035034
• Organism: Pantoea ananatis strain NN08200

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EPK90_RS09545
• Protein ID: WP_015700048.1
• Coordinates: 2013109..2013441 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EPK90_RS09540
• Protein ID: WP_014605554.1
• Coordinates: 2012851..2013108 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPK90_RS09505	2008059..2008574	+	516	WP_128391996.1	pentapeptide repeat-containing protein	-
EPK90_RS09510	2008850..2010088	-	1239	WP_014605550.1	MFS transporter	-
EPK90_RS09520	2010835..2011797	+	963	WP_042678147.1	ferrochelatase	-
EPK90_RS09530	2012298..2012503	+	206	Protein_1828	helix-turn-helix domain-containing protein	-
EPK90_RS24815	2012562..2012753	-	192	WP_081091687.1	hypothetical protein	-
EPK90_RS09540	2012851..2013108	+	258	WP_014605554.1	antitoxin	Antitoxin
EPK90_RS09545	2013109..2013441	+	333	WP_015700048.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EPK90_RS09555	2013702..2014535	-	834	WP_013026205.1	DgsA anti-repressor MtfA	-
EPK90_RS09560	2014641..2015318	-	678	WP_013026204.1	hypothetical protein	-
EPK90_RS09565	2015930..2016634	+	705	WP_014605556.1	phosphohydrolase	-
EPK90_RS09570	2016708..2016962	+	255	WP_013026202.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11945.66 Da
• Isoelectric Point: 6.9807

>T117140 WP_015700048.1 NZ_CP035034:2013109-2013441 [Pantoea ananatis]
MERGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T117140 NZ_CP035034:2013109-2013441 [Pantoea ananatis]
ATGGAAAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGGATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCATT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGAATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9253.73 Da
• Isoelectric Point: 4.5821

>AT117140 WP_014605554.1 NZ_CP035034:2012851-2013108 [Pantoea ananatis]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT117140 NZ_CP035034:2012851-2013108 [Pantoea ananatis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCTCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATTAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGCTCCTGTAG