Detailed information of TA system TA117130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 168486..169000
• Replicon: chromosome
• Accession: NZ_CP035005
• Organism: Staphylococcus aureus strain PCFH-226

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: EMO94_RS00955
• Protein ID: WP_000581792.1
• Coordinates: 168791..169000 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: EMO94_RS00950
• Protein ID: WP_000138932.1
• Coordinates: 168486..168779 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMO94_RS00930	164249..165564	+	1316	Protein_168	ISL3 family transposase	-
EMO94_RS00935	165561..165881	-	321	WP_001825748.1	hypothetical protein	-
EMO94_RS00940	166161..166808	-	648	WP_001187614.1	HD domain-containing protein	-
EMO94_RS00945	166836..168320	-	1485	WP_000571550.1	cardiolipin synthase	-
EMO94_RS00950	168486..168779	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
EMO94_RS00955	168791..169000	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
EMO94_RS00960	169128..169265	+	138	WP_000828354.1	hypothetical protein	-
EMO94_RS00965	169529..170731	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
EMO94_RS00970	171049..172119	+	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
EMO94_RS00975	172134..173489	+	1356	WP_128382619.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T117130 WP_000581792.1 NZ_CP035005:168791-169000 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T117130 NZ_CP035005:168791-169000 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT117130 WP_000138932.1 NZ_CP035005:168486-168779 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT117130 NZ_CP035005:168486-168779 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13