Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 918795..919309 Replicon chromosome
Accession NZ_CP035003
Organism Staphylococcus aureus strain PCFA-221

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag EMO93_RS04605 Protein ID WP_000581792.1
Coordinates 918795..919004 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag EMO93_RS04610 Protein ID WP_000138932.1
Coordinates 919016..919309 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EMO93_RS04585 914306..915661 - 1356 WP_128382619.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
EMO93_RS04590 915676..916746 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
EMO93_RS04595 917064..918266 + 1203 WP_001109939.1 rod shape-determining protein RodA -
EMO93_RS04600 918530..918667 - 138 WP_000828354.1 hypothetical protein -
EMO93_RS04605 918795..919004 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
EMO93_RS04610 919016..919309 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
EMO93_RS04615 919475..920959 + 1485 WP_000571550.1 cardiolipin synthase -
EMO93_RS04620 920987..921634 + 648 WP_001187614.1 HD domain-containing protein -
EMO93_RS04625 921914..922234 + 321 WP_001825748.1 hypothetical protein -
EMO93_RS04630 922231..923546 - 1316 Protein_845 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T117120 WP_000581792.1 NZ_CP035003:c919004-918795 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T117120 NZ_CP035003:c919004-918795 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT117120 WP_000138932.1 NZ_CP035003:c919309-919016 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT117120 NZ_CP035003:c919309-919016 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References