Detailed information of TA system TA117120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 918795..919309
• Replicon: chromosome
• Accession: NZ_CP035003
• Organism: Staphylococcus aureus strain PCFA-221

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: EMO93_RS04605
• Protein ID: WP_000581792.1
• Coordinates: 918795..919004 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: EMO93_RS04610
• Protein ID: WP_000138932.1
• Coordinates: 919016..919309 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EMO93_RS04585	914306..915661	-	1356	WP_128382619.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
EMO93_RS04590	915676..916746	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
EMO93_RS04595	917064..918266	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
EMO93_RS04600	918530..918667	-	138	WP_000828354.1	hypothetical protein	-
EMO93_RS04605	918795..919004	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
EMO93_RS04610	919016..919309	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
EMO93_RS04615	919475..920959	+	1485	WP_000571550.1	cardiolipin synthase	-
EMO93_RS04620	920987..921634	+	648	WP_001187614.1	HD domain-containing protein	-
EMO93_RS04625	921914..922234	+	321	WP_001825748.1	hypothetical protein	-
EMO93_RS04630	922231..923546	-	1316	Protein_845	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T117120 WP_000581792.1 NZ_CP035003:c919004-918795 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T117120 NZ_CP035003:c919004-918795 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT117120 WP_000138932.1 NZ_CP035003:c919309-919016 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT117120 NZ_CP035003:c919309-919016 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13