Detailed information of TA system TA117116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 435819..436504
• Replicon: plasmid pRapFH23d
• Accession: NZ_CP035002
• Organism: Rhizobium acidisoli strain FH23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CO657_RS34410
• Protein ID: WP_054183608.1
• Coordinates: 436082..436504 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CO657_RS34405
• Protein ID: WP_054183607.1
• Coordinates: 435819..436085 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO657_RS34385	431494..432183	+	690	WP_054183603.1	ABC transporter permease	-
CO657_RS34390	432290..433198	+	909	WP_054183604.1	homocysteine S-methyltransferase family protein	-
CO657_RS34395	433217..433654	-	438	WP_054183605.1	MarR family transcriptional regulator	-
CO657_RS34400	433861..435672	+	1812	WP_054183606.1	chloride channel protein	-
CO657_RS34405	435819..436085	+	267	WP_054183607.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CO657_RS34410	436082..436504	+	423	WP_054183608.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO657_RS34415	436544..437314	-	771	WP_054183609.1	ABC transporter ATP-binding protein	-
CO657_RS34420	437324..438307	-	984	WP_054183610.1	ABC transporter substrate-binding protein	-
CO657_RS34425	438346..439200	-	855	WP_054183611.1	ABC transporter permease	-
CO657_RS34430	439211..440563	-	1353	WP_054183612.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	gmd	1..845857	845857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-56)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15682.94 Da
• Isoelectric Point: 6.2462

>T117116 WP_054183608.1 NZ_CP035002:436082-436504 [Rhizobium acidisoli]
VKLLLDTNVLSEVTKPNPAERVLEWLDKLDEDRSFISIVSVAEIQRGIALMDKGRKRDALAEWLAHDLTQRFEHRIIPVD
EPVALAWGDLMGQAKRRGRGRSSMGGLIAANAVAHDLSLATRNTRDFEGFGVELIDPWAR

Nucleotide

• Length: 423 bp

>T117116 NZ_CP035002:436082-436504 [Rhizobium acidisoli]
GTGAAGCTTCTGCTCGACACCAATGTCCTGTCCGAGGTGACGAAGCCGAACCCTGCTGAGCGCGTCTTGGAGTGGCTGGA
CAAGCTCGACGAGGATCGCAGCTTCATCAGCATCGTGTCGGTCGCAGAGATCCAGCGCGGTATTGCCCTGATGGACAAGG
GACGGAAACGCGATGCGCTGGCCGAATGGCTTGCCCACGACCTGACGCAACGCTTCGAGCATCGCATCATTCCGGTGGAC
GAGCCGGTTGCCCTGGCATGGGGAGACCTTATGGGCCAGGCGAAGCGGAGGGGCCGCGGCCGGTCGTCGATGGGCGGACT
TATCGCTGCGAACGCGGTTGCTCACGATCTCAGCCTCGCGACGCGTAACACGAGGGATTTCGAGGGCTTTGGGGTCGAAC
TCATCGACCCCTGGGCCAGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9878.13 Da
• Isoelectric Point: 6.3725

>AT117116 WP_054183607.1 NZ_CP035002:435819-436085 [Rhizobium acidisoli]
MRLPKQGDESWTVAGAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARKGTLAEFLMESPFRGADLDLERQR
DEPRDPDL

Nucleotide

• Length: 267 bp

>AT117116 NZ_CP035002:435819-436085 [Rhizobium acidisoli]
ATGCGGCTACCAAAGCAAGGTGATGAAAGCTGGACTGTAGCCGGTGCAAAGGCGAAGCTTTCCGAGGTGATCGAACGCGC
GCAATCCGCGCCACAGACCATCACACGAAACGGCAAACCCAGCGTGGTCGTCGTCTCCGCCGAGGAATGGCAGCGGAAGA
CGGCACGCAAGGGGACGCTTGCCGAATTCTTGATGGAATCGCCGTTTCGCGGCGCCGATCTGGACCTTGAGCGTCAGCGC
GACGAACCGCGTGATCCTGACCTGTGA