Detailed information of TA system TA117115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 700880..701424
• Replicon: plasmid pRapFH23c
• Accession: NZ_CP035001
• Organism: Rhizobium acidisoli strain FH23

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CO657_RS32410
• Protein ID: WP_054184593.1
• Coordinates: 700880..701203 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CO657_RS32415
• Protein ID: WP_054184592.1
• Coordinates: 701200..701424 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO657_RS32385	697464..698261	-	798	WP_054184596.1	pilus assembly protein	-
CO657_RS32390	698267..698830	-	564	WP_054184595.1	A24 family peptidase	-
CO657_RS32395	698845..699048	-	204	WP_003563674.1	hypothetical protein	-
CO657_RS32405	699690..700832	+	1143	WP_054184594.1	CpaF family protein	-
CO657_RS32410	700880..701203	-	324	WP_054184593.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CO657_RS32415	701200..701424	-	225	WP_054184592.1	antitoxin MazE family protein	Antitoxin
CO657_RS32420	701546..702094	-	549	WP_054184591.1	sarcosine oxidase subunit gamma	-
CO657_RS32425	702087..705041	-	2955	WP_054184590.1	sarcosine oxidase subunit alpha family protein	-
CO657_RS32430	705038..705319	-	282	WP_054184589.1	sarcosine oxidase subunit delta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..709381	709381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11408.44 Da
• Isoelectric Point: 10.2912

>T117115 WP_054184593.1 NZ_CP035001:c701203-700880 [Rhizobium acidisoli]
VRRGDIVTVAITGDFGKPRPALIIQADQFDATGTVTVLLISGTLIDAPIIRPTIEPTALSGLRKVSQVMVDKTMSVKRDR
IGPIIGRLEDDALLAVTRSLAVFLSIA

Nucleotide

• Length: 324 bp

>T117115 NZ_CP035001:c701203-700880 [Rhizobium acidisoli]
GTGAGGCGCGGTGACATCGTAACCGTCGCAATCACCGGCGATTTCGGCAAGCCTCGTCCGGCGCTTATCATCCAGGCAGA
TCAATTCGACGCAACGGGCACGGTTACCGTGCTGCTGATTTCTGGCACGCTCATCGATGCGCCGATCATTCGGCCAACCA
TCGAGCCGACAGCACTCAGCGGTTTGCGCAAAGTCTCGCAAGTGATGGTGGATAAAACCATGTCCGTGAAACGGGATAGA
ATTGGCCCGATAATCGGCCGCCTGGAAGATGATGCTCTCCTGGCGGTCACAAGATCGCTCGCCGTTTTTCTGAGTATCGC
CTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8158.05 Da
• Isoelectric Point: 4.5720

>AT117115 WP_054184592.1 NZ_CP035001:c701424-701200 [Rhizobium acidisoli]
MATPVNERVQKRREALRAAGLRPVQIWVPDTRRPGFAEECHRQSVQAALADAGDRDLQDFLDGALADLDSEGEA

Nucleotide

• Length: 225 bp

>AT117115 NZ_CP035001:c701424-701200 [Rhizobium acidisoli]
ATGGCAACGCCGGTCAACGAAAGAGTTCAAAAGCGCCGCGAGGCCTTGCGGGCTGCTGGGCTGCGTCCCGTGCAAATATG
GGTGCCCGATACCCGCCGCCCCGGTTTTGCCGAGGAATGCCATCGTCAGTCCGTTCAGGCAGCCCTTGCCGACGCCGGAG
ATCGCGATTTGCAGGATTTCCTCGATGGCGCACTCGCCGATCTGGACTCCGAAGGTGAGGCGTGA