Detailed information of TA system TA117114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 363104..363684
• Replicon: plasmid pRapFH23c
• Accession: NZ_CP035001
• Organism: Rhizobium acidisoli strain FH23

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CO657_RS30735
• Protein ID: WP_054184627.1
• Coordinates: 363301..363684 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0N1DLL7
• Locus tag: CO657_RS30730
• Protein ID: WP_054184628.1
• Coordinates: 363104..363304 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO657_RS30685	358615..359601	+	987	WP_054184632.1	AraC family transcriptional regulator	-
CO657_RS30690	359697..359897	+	201	Protein_340	nitroreductase family protein	-
CO657_RS30700	360039..360539	+	501	WP_097609960.1	NAD(P)H-dependent oxidoreductase	-
CO657_RS30705	360653..360814	+	162	WP_128715642.1	DUF3788 family protein	-
CO657_RS30710	360950..361867	+	918	WP_054184631.1	NADP-dependent oxidoreductase	-
CO657_RS30715	362071..362361	+	291	WP_054184724.1	hypothetical protein	-
CO657_RS30720	362424..362639	-	216	WP_054184630.1	hypothetical protein	-
CO657_RS30730	363104..363304	+	201	WP_054184628.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CO657_RS30735	363301..363684	+	384	WP_054184627.1	type II toxin-antitoxin system VapC family toxin	Toxin
CO657_RS30740	364252..364515	+	264	WP_054184626.1	hypothetical protein	-
CO657_RS30745	364594..364821	-	228	Protein_349	DUF2243 domain-containing protein	-
CO657_RS30750	364857..365117	-	261	WP_082366318.1	DUF982 domain-containing protein	-
CO657_RS30755	365653..365943	-	291	WP_054184624.1	hypothetical protein	-
CO657_RS30760	366038..366310	-	273	WP_054184623.1	aspartate-semialdehyde dehydrogenase	-
CO657_RS30765	366587..366901	+	315	WP_054184622.1	co-chaperone GroES	-
CO657_RS30770	366956..368584	+	1629	WP_054184621.1	chaperonin GroEL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..709381	709381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14129.17 Da
• Isoelectric Point: 6.3741

>T117114 WP_054184627.1 NZ_CP035001:363301-363684 [Rhizobium acidisoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLGDRSSVIAFLAAQREAFVATHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNPRN

Nucleotide

• Length: 384 bp

>T117114 NZ_CP035001:363301-363684 [Rhizobium acidisoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
CCGTCTCTTATGCCATCCAGCCGTAATCGGAGAGCTCGCACTTGGCAGCCTTGGGGATCGCAGTAGTGTGATAGCATTCC
TGGCGGCCCAGCGCGAAGCGTTCGTCGCGACGCACGACGAAGTTATGATGATGATTGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGACCAACGAGCGGCGCTGTGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACCCCTGACAACCCGCGAAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7181.37 Da
• Isoelectric Point: 11.7627

>AT117114 WP_054184628.1 NZ_CP035001:363104-363304 [Rhizobium acidisoli]
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAKAAPRRRSVAAK

Nucleotide

• Length: 201 bp

>AT117114 NZ_CP035001:363104-363304 [Rhizobium acidisoli]
ATGCGATCGACTATCAATCTCGACGACACCCTTATGGAAAGGGCTAGGTCTCTGACGGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTAATCGCGCTCGGCGGCACTATGCCGGATG
CGAAAGCGGCTCCACGCCGCCGGAGCGTAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1DLL7