Detailed information of TA system TA117092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1328670..1329295
• Replicon: chromosome
• Accession: NZ_CP034966
• Organism: Escherichia coli strain WCHEC020032

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7V17_RS08315
• Protein ID: WP_000911330.1
• Coordinates: 1328897..1329295 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C7V17_RS08310
• Protein ID: WP_000450524.1
• Coordinates: 1328670..1328897 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V17_RS08285	1324473..1324943	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C7V17_RS08290	1324943..1325515	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C7V17_RS08295	1325661..1326539	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C7V17_RS08300	1326556..1327590	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C7V17_RS08305	1327803..1328516	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C7V17_RS08310	1328670..1328897	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C7V17_RS08315	1328897..1329295	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C7V17_RS08320	1329442..1330305	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
C7V17_RS08325	1330320..1332335	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
C7V17_RS08330	1332409..1333107	+	699	WP_000679823.1	esterase	-
C7V17_RS08335	1333217..1333417	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T117092 WP_000911330.1 NZ_CP034966:1328897-1329295 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T117092 NZ_CP034966:1328897-1329295 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT117092 WP_000450524.1 NZ_CP034966:1328670-1328897 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT117092 NZ_CP034966:1328670-1328897 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33