Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 63115..63716 Replicon plasmid p1_020032
Accession NZ_CP034959
Organism Escherichia coli strain WCHEC020032

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag C7V17_RS00320 Protein ID WP_001216045.1
Coordinates 63115..63495 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag C7V17_RS00325 Protein ID WP_001190712.1
Coordinates 63495..63716 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V17_RS00295 58556..60040 - 1485 WP_000124150.1 terminase -
C7V17_RS00300 60040..61233 - 1194 WP_000219625.1 hypothetical protein -
C7V17_RS00305 61319..61771 - 453 WP_001326849.1 late promoter-activating protein -
C7V17_RS00310 61860..62903 - 1044 WP_023356283.1 DUF968 domain-containing protein -
C7V17_RS00315 62931..63110 - 180 WP_001339207.1 hypothetical protein -
C7V17_RS00320 63115..63495 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
C7V17_RS00325 63495..63716 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C7V17_RS00330 63789..64178 - 390 WP_000506730.1 DNA repair protein -
C7V17_RS00335 64353..64568 + 216 Protein_60 DNA-binding protein -
C7V17_RS00340 64570..64944 + 375 Protein_61 integrase core domain-containing protein -
C7V17_RS00345 65515..67371 - 1857 WP_077902027.1 acyltransferase -
C7V17_RS00350 67597..68343 + 747 Protein_63 IS1 family transposase -
C7V17_RS24835 68442..68621 - 180 Protein_64 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..90842 90842
- flank IS/Tn - - 64570..64944 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T117077 WP_001216045.1 NZ_CP034959:c63495-63115 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T117077 NZ_CP034959:c63495-63115 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT117077 WP_001190712.1 NZ_CP034959:c63716-63495 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT117077 NZ_CP034959:c63716-63495 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References