Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3511197..3511815 Replicon chromosome
Accession NZ_CP034958
Organism Escherichia coli strain SCEC020026

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C7V14_RS18120 Protein ID WP_001291435.1
Coordinates 3511597..3511815 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C7V14_RS18115 Protein ID WP_000344800.1
Coordinates 3511197..3511571 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V14_RS18105 3506286..3507479 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C7V14_RS18110 3507502..3510651 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C7V14_RS18115 3511197..3511571 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C7V14_RS18120 3511597..3511815 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C7V14_RS18125 3511987..3512538 + 552 WP_000102568.1 maltose O-acetyltransferase -
C7V14_RS18130 3512654..3513103 + 450 Protein_3300 YlaC family protein -
C7V14_RS18135 3513287..3514837 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C7V14_RS18140 3514879..3515232 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C7V14_RS18150 3515611..3515922 + 312 WP_000409911.1 MGMT family protein -
C7V14_RS18155 3515953..3516525 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T117075 WP_001291435.1 NZ_CP034958:3511597-3511815 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T117075 NZ_CP034958:3511597-3511815 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT117075 WP_000344800.1 NZ_CP034958:3511197-3511571 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT117075 NZ_CP034958:3511197-3511571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References