Detailed information of TA system TA117041

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3551244..3551923
• Replicon: chromosome
• Accession: NZ_CP034953
• Organism: Escherichia coli strain MT102

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: EPB33_RS17355
• Protein ID: WP_000854672.1
• Coordinates: 3551582..3551923 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: EPB33_RS17350
• Protein ID: WP_000070395.1
• Coordinates: 3551244..3551561 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPB33_RS17300	3546291..3547154	+	864	WP_001065553.1	GTPase family protein	-
EPB33_RS17305	3547246..3548067	+	822	WP_000197389.1	DUF945 domain-containing protein	-
EPB33_RS17310	3548284..3548475	+	192	Protein_3375	DeoR family transcriptional regulator	-
EPB33_RS17315	3548471..3548698	+	228	WP_001548158.1	hypothetical protein	-
EPB33_RS17320	3548698..3549141	+	444	WP_000824223.1	hypothetical protein	-
EPB33_RS17325	3549164..3549631	+	468	WP_001547765.1	hypothetical protein	-
EPB33_RS17330	3549708..3549947	+	240	WP_000194654.1	DUF905 domain-containing protein	-
EPB33_RS17335	3550045..3550503	+	459	WP_000211838.1	antirestriction protein	-
EPB33_RS17340	3550519..3550995	+	477	WP_000811693.1	RadC family protein	-
EPB33_RS17345	3551004..3551225	+	222	WP_000691994.1	DUF987 domain-containing protein	-
EPB33_RS17350	3551244..3551561	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
EPB33_RS17355	3551582..3551923	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
EPB33_RS17365	3552495..3553748	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
EPB33_RS17370	3553760..3554863	-	1104	WP_001285288.1	glutamate 5-kinase	-
EPB33_RS17375	3555151..3556206	+	1056	WP_000749863.1	phosphoporin PhoE	-
EPB33_RS17380	3556245..3556646	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3535946..3552143	16197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T117041 WP_000854672.1 NZ_CP034953:3551582-3551923 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T117041 NZ_CP034953:3551582-3551923 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT117041 WP_000070395.1 NZ_CP034953:3551244-3551561 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT117041 NZ_CP034953:3551244-3551561 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684