Detailed information of TA system TA117028

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1803122..1803953
• Replicon: chromosome
• Accession: NZ_CP034953
• Organism: Escherichia coli strain MT102

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: EPB33_RS08580
• Protein ID: WP_000854814.1
• Coordinates: 1803122..1803496 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: EPB33_RS08585
• Protein ID: WP_001285584.1
• Coordinates: 1803585..1803953 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPB33_RS08540	1798518..1799684	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
EPB33_RS08545	1799803..1800276	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
EPB33_RS08550	1800474..1801532	+	1059	WP_001200891.1	FUSC family protein	-
EPB33_RS08555	1801704..1802033	+	330	WP_000450409.1	DUF496 family protein	-
EPB33_RS08560	1802134..1802316	-	183	WP_001016348.1	hypothetical protein	-
EPB33_RS08565	1802388..1802516	+	129	Protein_1675	transposase domain-containing protein	-
EPB33_RS08570	1802805..1802918	-	114	WP_001161660.1	DUF957 domain-containing protein	-
EPB33_RS08575	1802931..1803125	-	195	WP_000988600.1	hypothetical protein	-
EPB33_RS08580	1803122..1803496	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
EPB33_RS08585	1803585..1803953	-	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EPB33_RS08590	1804027..1804248	-	222	WP_000692323.1	DUF987 domain-containing protein	-
EPB33_RS08595	1804311..1804757	-	447	WP_000187523.1	RadC family protein	-
EPB33_RS08600	1804754..1806286	-	1533	WP_001350525.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T117028 WP_000854814.1 NZ_CP034953:c1803496-1803122 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T117028 NZ_CP034953:c1803496-1803122 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT117028 WP_001285584.1 NZ_CP034953:c1803953-1803585 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT117028 NZ_CP034953:c1803953-1803585 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6