Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 1544225..1544754 Replicon chromosome
Accession NZ_CP034949
Organism Enterococcus faecium strain NM213

Toxin (Protein)


Gene name relE Uniprot ID C9B8W9
Locus tag EO217_RS09295 Protein ID WP_002294198.1
Coordinates 1544225..1544491 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C9B8W8
Locus tag EO217_RS09300 Protein ID WP_002294199.1
Coordinates 1544491..1544754 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO217_RS09270 1539592..1540767 + 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -
EO217_RS09275 1540771..1541412 + 642 WP_002293405.1 ABC transporter permease -
EO217_RS09280 1541409..1542326 + 918 WP_002294193.1 osmoprotectant ABC transporter substrate-binding protein -
EO217_RS09285 1542323..1542994 + 672 WP_002319469.1 ABC transporter permease -
EO217_RS15040 1543306..1543473 - 168 WP_002294195.1 hypothetical protein -
EO217_RS09290 1543536..1544024 - 489 WP_136977653.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
EO217_RS09295 1544225..1544491 - 267 WP_002294198.1 Txe/YoeB family addiction module toxin Toxin
EO217_RS09300 1544491..1544754 - 264 WP_002294199.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EO217_RS09310 1545229..1545414 + 186 WP_002294201.1 hypothetical protein -
EO217_RS09315 1545556..1547379 - 1824 WP_002294202.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(22-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10646.11 Da        Isoelectric Point: 9.9931

>T117015 WP_002294198.1 NZ_CP034949:c1544491-1544225 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T117015 NZ_CP034949:c1544491-1544225 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10016.03 Da        Isoelectric Point: 4.1207

>AT117015 WP_002294199.1 NZ_CP034949:c1544754-1544491 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Download         Length: 264 bp

>AT117015 NZ_CP034949:c1544754-1544491 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FMA0

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZU84

References