Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1255768..1256339 Replicon chromosome
Accession NZ_CP034949
Organism Enterococcus faecium strain NM213

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EO217_RS07865 Protein ID WP_002286801.1
Coordinates 1255768..1256109 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EO217_RS07870 Protein ID WP_002323011.1
Coordinates 1256109..1256339 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO217_RS07835 1250978..1252312 - 1335 WP_002333419.1 ABC transporter substrate-binding protein -
EO217_RS07840 1252520..1253350 + 831 WP_002286789.1 manganese catalase family protein -
EO217_RS07850 1253594..1253876 - 283 Protein_1191 transposase -
EO217_RS07855 1254034..1254228 - 195 WP_002295146.1 hypothetical protein -
EO217_RS07860 1254733..1254918 - 186 WP_002304207.1 hypothetical protein -
EO217_RS07865 1255768..1256109 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EO217_RS07870 1256109..1256339 - 231 WP_002323011.1 hypothetical protein Antitoxin
EO217_RS07880 1256626..1256964 + 339 WP_002286804.1 hypothetical protein -
EO217_RS07885 1257169..1257744 - 576 WP_136977641.1 SOS response-associated peptidase family protein -
EO217_RS07900 1258353..1258934 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EO217_RS07905 1259117..1259743 - 627 WP_002333420.1 cysteine hydrolase -
EO217_RS07910 1259765..1261093 - 1329 WP_008265691.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T117014 WP_002286801.1 NZ_CP034949:c1256109-1255768 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T117014 NZ_CP034949:c1256109-1255768 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT117014 WP_002323011.1 NZ_CP034949:c1256339-1256109 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT117014 NZ_CP034949:c1256339-1256109 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References