Detailed information of TA system TA117002

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1886804..1887527
• Replicon: chromosome
• Accession: NZ_CP034938
• Organism: Pectobacterium odoriferum strain JK2.1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EO763_RS08360
• Protein ID: WP_161528671.1
• Coordinates: 1887207..1887527 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EO763_RS08355
• Protein ID: WP_161528670.1
• Coordinates: 1886804..1887139 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO763_RS08330	1882519..1883451	+	933	WP_161528666.1	hypothetical protein	-
EO763_RS08335	1883547..1884434	+	888	WP_161528667.1	50S ribosome-binding GTPase	-
EO763_RS23260	1884618..1884776	+	159	WP_154715508.1	hypothetical protein	-
EO763_RS08340	1884923..1885756	+	834	WP_161528668.1	hypothetical protein	-
EO763_RS08345	1885813..1886163	+	351	WP_116156686.1	hypothetical protein	-
EO763_RS08350	1886291..1886764	+	474	WP_161528669.1	DNA repair protein RadC	-
EO763_RS08355	1886804..1887139	+	336	WP_161528670.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EO763_RS08360	1887207..1887527	+	321	WP_161528671.1	TA system toxin CbtA family protein	Toxin
EO763_RS08365	1887645..1888478	+	834	WP_161528672.1	DUF4942 domain-containing protein	-
EO763_RS08370	1888572..1888802	+	231	WP_132455036.1	antitoxin	-
EO763_RS08375	1888802..1889206	+	405	WP_161528673.1	tRNA(fMet)-specific endonuclease VapC	-
EO763_RS08380	1889512..1889751	-	240	Protein_1631	integrase	-
EO763_RS08385	1889810..1890685	-	876	WP_161528674.1	HNH endonuclease	-
EO763_RS08390	1890774..1891319	-	546	WP_161528675.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1872073..1895037	22964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12074.81 Da
• Isoelectric Point: 5.0744

>T117002 WP_161528671.1 NZ_CP034938:1887207-1887527 [Pectobacterium odoriferum]
MQTTPAIPPREDNPCASPIAVWQQLLTYLLEKHYGLTLNDTPFCEEHVIQEHIDAGVTLLNAVNFLVEKYELVRIDRNGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T117002 NZ_CP034938:1887207-1887527 [Pectobacterium odoriferum]
ATGCAAACAACACCTGCAATCCCGCCACGGGAGGATAACCCCTGTGCGTCGCCTATAGCCGTCTGGCAGCAGCTTCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTGACGCTAAACGACACACCGTTCTGCGAGGAACATGTGATTCAGGAACATATTG
ATGCTGGCGTCACGTTGCTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAATGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTCACTGCTGTTGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAAGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12461.05 Da
• Isoelectric Point: 5.1365

>AT117002 WP_161528670.1 NZ_CP034938:1886804-1887139 [Pectobacterium odoriferum]
MSSSDTPEWGLKCTITPRFGARLVQEGNRLHYLADRDSVVGTFSKTEAQSLERCFSELIKQLEQKLRTGELNPRQQGCVT
LHCNEWTCEADTLGSVGYVYIVIYPTSAVTE

Nucleotide

• Length: 336 bp

>AT117002 NZ_CP034938:1886804-1887139 [Pectobacterium odoriferum]
ATGTCATCATCGGATACCCCGGAATGGGGATTAAAATGTACCATCACGCCGCGATTTGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGCAGACCGGGACAGCGTTGTCGGTACGTTCAGCAAAACGGAAGCCCAAAGTCTGGAACGTT
GCTTCTCTGAGCTGATCAAGCAGTTGGAGCAGAAACTACGCACGGGGGAACTGAATCCCCGCCAGCAGGGCTGTGTTACG
TTGCATTGCAACGAATGGACCTGTGAAGCCGATACGCTGGGCAGCGTTGGCTACGTGTATATCGTTATTTATCCAACTTC
TGCGGTAACAGAATAA