Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 2869727..2870364 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P95004 |
| Locus tag | Rv2549c | Protein ID | NP_217065.1 |
| Coordinates | 2869727..2870122 (-) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ45 |
| Locus tag | Rv2550c | Protein ID | NP_217066.1 |
| Coordinates | 2870119..2870364 (-) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv2542 | 2865130..2866341 | + | 1212 | NP_217058.1 | hypothetical protein | - |
| Rv2543 | 2866468..2867127 | + | 660 | NP_217059.1 | lipoprotein LppA | - |
| Rv2544 | 2867124..2867786 | + | 663 | NP_217060.1 | lipoprotein LppB | - |
| Rv2545 | 2867783..2868061 | + | 279 | NP_217061.1 | antitoxin VapB18 | - |
| Rv2546 | 2868154..2868567 | + | 414 | NP_217062.1 | ribonuclease VapC18 | - |
| Rv2547 | 2868606..2868863 | + | 258 | NP_217063.1 | antitoxin VapB19 | - |
| Rv2548 | 2868860..2869237 | + | 378 | NP_217064.1 | ribonuclease VapC19 | - |
| Rv2548A | 2869253..2869627 | - | 375 | YP_004837057.1 | hypothetical protein | - |
| Rv2549c | 2869727..2870122 | - | 396 | NP_217065.1 | ribonuclease VapC20 | Toxin |
| Rv2550c | 2870119..2870364 | - | 246 | NP_217066.1 | antitoxin VapB20 | Antitoxin |
| Rv2551c | 2870775..2871194 | - | 420 | NP_217067.1 | hypothetical protein | - |
| Rv2552c | 2871206..2872015 | - | 810 | NP_217068.1 | shikimate 5-dehydrogenase | - |
| Rv2553c | 2872012..2873265 | - | 1254 | NP_217069.1 | membrane protein | - |
| Rv2554c | 2873258..2873770 | - | 513 | NP_217070.1 | Holliday junction resolvase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 14619.53 Da Isoelectric Point: 6.9794
>T117 NP_217065.1 NC_000962:c2870122-2869727 [Mycobacterium tuberculosis H37Rv]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
Download Length: 396 bp
>T117 NC_000962:c2870122-2869727 [Mycobacterium tuberculosis H37Rv]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
Antitoxin
Download Length: 82 a.a. Molecular weight: 9098.33 Da Isoelectric Point: 5.6007
>AT117 NP_217066.1 NC_000962:c2870364-2870119 [Mycobacterium tuberculosis H37Rv]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
Download Length: 246 bp
>AT117 NC_000962:c2870364-2870119 [Mycobacterium tuberculosis H37Rv]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Kristoffer S Winther et al. (2013) VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S rRNA. Nature Communications 4:2796. [PubMed:24225902]
(2) Amar Deep et al. (2017) Crystal structure of Mycobacterium tuberculosis VapC20 toxin and its interactions with cognate antitoxin, VapB20, suggest a model for toxin-antitoxin assembly. The FEBS Journal 284(23):4066-4082. [PubMed:28986943]
(3) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]