Detailed information of TA system TA116995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 433708..434547
• Replicon: chromosome
• Accession: NZ_CP034938
• Organism: Pectobacterium odoriferum strain JK2.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7V8PT96
• Locus tag: EO763_RS01940
• Protein ID: WP_039535732.1
• Coordinates: 434185..434547 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EO763_RS01935
• Protein ID: WP_161527908.1
• Coordinates: 433708..434181 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO763_RS01915	428813..429736	-	924	WP_161527905.1	aminoimidazole riboside kinase	-
EO763_RS01920	430060..430248	+	189	WP_039470389.1	hypothetical protein	-
EO763_RS01925	430418..430969	+	552	WP_161527906.1	polymer-forming cytoskeletal protein	-
EO763_RS01930	431001..433718	+	2718	WP_161527907.1	CRISPR-associated helicase Cas3'	-
EO763_RS01935	433708..434181	-	474	WP_161527908.1	helix-turn-helix domain-containing protein	Antitoxin
EO763_RS01940	434185..434547	-	363	WP_039535732.1	type II toxin-antitoxin system HigB family toxin	Toxin
EO763_RS01945	435161..436729	+	1569	WP_161527909.1	type I-E CRISPR-associated protein Cse1/CasA	-
EO763_RS01950	436713..437360	+	648	WP_161527910.1	type I-E CRISPR-associated protein Cse2/CasB	-
EO763_RS01955	437364..438431	+	1068	WP_161527911.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
EO763_RS01960	438441..439175	+	735	WP_103183095.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (74-111)

Antitoxin

• Antitoxin HigA (43-157)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14161.38 Da
• Isoelectric Point: 9.7305

>T116995 WP_039535732.1 NZ_CP034938:c434547-434185 [Pectobacterium odoriferum]
MRVISAKAITDAMKKHAKWKVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFALLENESRKGPLDIYVFDIN
GNNCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T116995 NZ_CP034938:c434547-434185 [Pectobacterium odoriferum]
ATGAGAGTCATCTCAGCCAAGGCCATCACCGATGCGATGAAAAAGCATGCGAAATGGAAAGTTGGTTTGAAGTTATGGCT
GGATGTTTTTGACAAGCCATCGCTGCGGTTTGAGTCCTACGAACAGTTATGTCATGTATGGCGAAATACATCTTGCTGGA
ATGTCGATCGCATACCTTTCGCATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGATATATATGTATTTGATATTAAT
GGTAACAATTGCAGAATCATAGCATGGTTAAACCCTAATACGGGTTCTTTGTATGTGAAAGACGTGTGCTCTCACGCAGA
GTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17805.10 Da
• Isoelectric Point: 4.2775

>AT116995 WP_161527908.1 NZ_CP034938:c434181-433708 [Pectobacterium odoriferum]
MRNTSLGSNRSVGISGLIENLPLLLQEASEQLNSLSVLLSACMEPIEDDNDLQERMECINQLYLYSSDVSDIPATFAELI
ADRVYEYEKKHRDVPHVSQADALAFFIEERGLKQSDLKQIATQSVISEIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT116995 NZ_CP034938:c434181-433708 [Pectobacterium odoriferum]
ATGCGAAATACATCTCTGGGTTCTAACCGTTCTGTAGGAATCAGTGGGCTCATCGAAAATTTACCTCTTCTGCTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTACTCTCTGCCTGTATGGAACCTATTGAAGACGATAACGATCTTCAGG
AAAGAATGGAGTGCATCAATCAACTTTACCTCTATTCCTCTGATGTCAGTGATATTCCAGCCACTTTTGCAGAGCTCATC
GCGGACAGAGTCTATGAATATGAAAAGAAACACCGAGATGTGCCCCACGTCAGTCAGGCTGATGCCTTAGCTTTTTTTAT
TGAAGAAAGAGGGCTGAAACAGTCAGACTTAAAGCAAATTGCAACACAAAGCGTCATCTCTGAGATTATTAACGGTAAGC
GAACCATGACATTACAGCAAGTTAAGGATTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA

Structures

Toxin

• AlphaFold DB: A0A7V8PT96