Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3818527..3819110 Replicon chromosome
Accession NZ_CP034935
Organism Shigella dysenteriae strain 79-8006

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag EPD85_RS20285 Protein ID WP_000254733.1
Coordinates 3818775..3819110 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EPD85_RS20280 Protein ID WP_000581937.1
Coordinates 3818527..3818775 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD85_RS20270 (3814866) 3814866..3816167 + 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
EPD85_RS20275 (3816215) 3816215..3818449 + 2235 WP_000226815.1 GTP pyrophosphokinase -
EPD85_RS20280 (3818527) 3818527..3818775 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EPD85_RS20285 (3818775) 3818775..3819110 + 336 WP_000254733.1 endoribonuclease MazF Toxin
EPD85_RS20290 (3819181) 3819181..3819972 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
EPD85_RS20295 (3820200) 3820200..3821837 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EPD85_RS20300 (3821925) 3821925..3823223 + 1299 WP_000036723.1 phosphopyruvate hydratase -
EPD85_RS20305 (3823283) 3823283..3823609 - 327 Protein_3850 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T116988 WP_000254733.1 NZ_CP034935:3818775-3819110 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T116988 NZ_CP034935:3818775-3819110 [Shigella dysenteriae]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT116988 WP_000581937.1 NZ_CP034935:3818527-3818775 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT116988 NZ_CP034935:3818527-3818775 [Shigella dysenteriae]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References