Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3026378..3027009 Replicon chromosome
Accession NZ_CP034935
Organism Shigella dysenteriae strain 79-8006

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag EPD85_RS16245 Protein ID WP_001259385.1
Coordinates 3026378..3026653 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3P6LLN4
Locus tag EPD85_RS16250 Protein ID WP_000593559.1
Coordinates 3026650..3027009 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD85_RS16230 (3021382) 3021382..3022449 + 1068 WP_000361465.1 HlyD family secretion protein -
EPD85_RS16235 (3022446) 3022446..3025181 + 2736 WP_000149136.1 ribosome-associated ATPase/putative transporter RbbA -
EPD85_RS16240 (3025181) 3025181..3026305 + 1125 WP_001216257.1 ABC transporter permease -
EPD85_RS16245 (3026378) 3026378..3026653 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
EPD85_RS16250 (3026650) 3026650..3027009 + 360 WP_000593559.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EPD85_RS16255 (3027129) 3027129..3027530 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EPD85_RS16260 (3027536) 3027536..3028342 - 807 WP_000173706.1 nickel import ATP-binding protein NikE -
EPD85_RS16265 (3028339) 3028339..3029103 - 765 WP_001136222.1 nickel import ATP-binding protein NikD -
EPD85_RS16270 (3029103) 3029103..3029936 - 834 WP_001008964.1 nickel ABC transporter permease subunit NikC -
EPD85_RS16275 (3029933) 3029933..3030877 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T116985 WP_001259385.1 NZ_CP034935:3026378-3026653 [Shigella dysenteriae]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T116985 NZ_CP034935:3026378-3026653 [Shigella dysenteriae]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13373.27 Da        Isoelectric Point: 4.8838

>AT116985 WP_000593559.1 NZ_CP034935:3026650-3027009 [Shigella dysenteriae]
MIKLKTPNTMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT116985 NZ_CP034935:3026650-3027009 [Shigella dysenteriae]
ATGATCAAACTGAAGACGCCAAACACTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A3P6LLN4

References