Detailed information of TA system TA116969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 47045..47820
• Replicon: plasmid p79-8006
• Accession: NZ_CP034934
• Organism: Shigella dysenteriae strain 79-8006

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q326V1
• Locus tag: EPD85_RS00295
• Protein ID: WP_000405248.1
• Coordinates: 47338..47820 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: EPD85_RS00290
• Protein ID: WP_010921625.1
• Coordinates: 47045..47347 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPD85_RS00270	43409..44863	+	1455	WP_000701106.1	type 3 secretion system effector OspC2	-
EPD85_RS00275	45012..45140	-	129	Protein_55	IS5/IS1182 family transposase	-
EPD85_RS00280	45312..46520	-	1209	WP_046891828.1	IS91-like element ISSbo1 family transposase	-
EPD85_RS00285	46501..46773	-	273	WP_000019158.1	hypothetical protein	-
EPD85_RS00290	47045..47347	+	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
EPD85_RS00295	47338..47820	+	483	WP_000405248.1	GNAT family N-acetyltransferase	Toxin
EPD85_RS00300	47888..48411	+	524	Protein_60	transposase	-
EPD85_RS00305	48454..48792	-	339	Protein_61	integrase core domain-containing protein	-
EPD85_RS00310	48866..50033	+	1168	Protein_62	IS3 family transposase	-
EPD85_RS00315	50057..50749	-	693	WP_000625268.1	type III secretion system effector cysteine methyltransferase OspZ	-
EPD85_RS00320	50805..50957	+	153	Protein_64	IS3 family transposase	-
EPD85_RS00325	51239..51376	-	138	WP_005026553.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI	1..100586	100586
	-	inside	IScluster/Tn	-	ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2	38363..82046	43683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17736.50 Da
• Isoelectric Point: 7.0382

>T116969 WP_000405248.1 NZ_CP034934:47338-47820 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T116969 NZ_CP034934:47338-47820 [Shigella dysenteriae]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT116969 WP_010921625.1 NZ_CP034934:47045-47347 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT116969 NZ_CP034934:47045-47347 [Shigella dysenteriae]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A090NWA7

Antitoxin

• AlphaFold DB: Q7BEG8