116967

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12901..13426 Replicon plasmid p79-8006
Accession NZ_CP034934
Organism Shigella dysenteriae strain 79-8006

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag EPD85_RS00100 Protein ID WP_001159860.1
Coordinates 13121..13426 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag EPD85_RS00095 Protein ID WP_000813626.1
Coordinates 12901..13119 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD85_RS00070 8198..9328 - 1131 WP_050546159.1 IS91 family transposase -
EPD85_RS00085 10700..10972 - 273 WP_000019158.1 hypothetical protein -
EPD85_RS00090 11429..12657 - 1229 Protein_16 IS3-like element IS2 family transposase -
EPD85_RS00095 12901..13119 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EPD85_RS00100 13121..13426 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
EPD85_RS00105 13450..13557 + 108 WP_023592908.1 transposase domain-containing protein -
EPD85_RS00110 13819..15456 + 1638 WP_129593020.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
EPD85_RS00115 15664..15933 + 270 WP_000079956.1 type II toxin-antitoxin system antitoxin YacA -
EPD85_RS24860 15933..16102 + 170 Protein_22 type II toxin-antitoxin system toxin YacB -
EPD85_RS00120 16185..16667 + 483 Protein_23 type III secretion system effector protein kinase OspG -
EPD85_RS24865 17434..17586 - 153 Protein_25 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH9.8 / ospG / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ospI 1..100586 100586
- inside IScluster/Tn - ipaH9.8 / ospG 5..23310 23305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T116967 WP_001159860.1 NZ_CP034934:13121-13426 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T116967 NZ_CP034934:13121-13426 [Shigella dysenteriae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT116967 WP_000813626.1 NZ_CP034934:12901-13119 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT116967 NZ_CP034934:12901-13119 [Shigella dysenteriae]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References