Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 35015..35772 Replicon plasmid p2013C-3749-2
Accession NZ_CP034933
Organism Shigella flexneri strain 2013C-3749

Toxin (Protein)


Gene name tacT Uniprot ID D2AJG5
Locus tag EPD86_RS24150 Protein ID WP_000501974.1
Coordinates 35287..35772 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag EPD86_RS24145 Protein ID WP_011114751.1
Coordinates 35015..35299 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD86_RS24125 31076..31750 + 675 WP_004967157.1 IS66-like element accessory protein TnpA -
EPD86_RS24130 31747..32097 + 351 WP_005048975.1 IS66 family insertion sequence element accessory protein TnpB -
EPD86_RS24135 32094..33695 + 1602 WP_005063916.1 IS66-like element ISSfl3 family transposase -
EPD86_RS24140 33949..34113 - 165 WP_001346193.1 hypothetical protein -
EPD86_RS24145 35015..35299 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
EPD86_RS24150 35287..35772 + 486 WP_000501974.1 GNAT family N-acetyltransferase Toxin
EPD86_RS24155 35945..36226 - 282 Protein_39 IS4/IS5 family transposase -
EPD86_RS24165 36293..37449 + 1157 WP_094085527.1 IS3-like element IS600 family transposase -
EPD86_RS24170 37519..38792 + 1274 WP_094081497.1 IS3-like element IS2 family transposase -
EPD86_RS24175 38830..38973 + 144 Protein_42 DUF3440 domain-containing protein -
EPD86_RS24180 38958..39596 + 639 WP_000502854.1 ParB N-terminal domain-containing protein -
EPD86_RS24185 39824..40248 + 425 Protein_44 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 / ospC2 / ipaH2.5 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospB / icsP/sopA / nleE / ospE1 / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC 1..223405 223405
- inside IScluster/Tn - ospC3 / ospC1 / ospD3/senA / ipaH4.5 / ipaH7.8 23086..66446 43360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17814.63 Da        Isoelectric Point: 9.8822

>T116961 WP_000501974.1 NZ_CP034933:35287-35772 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T116961 NZ_CP034933:35287-35772 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT116961 WP_011114751.1 NZ_CP034933:35015-35299 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT116961 NZ_CP034933:35015-35299 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PN28

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References