Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3255295..3255949 Replicon chromosome
Accession NZ_CP034931
Organism Shigella flexneri strain 2013C-3749

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag EPD86_RS16745 Protein ID WP_000244767.1
Coordinates 3255295..3255702 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag EPD86_RS16750 Protein ID WP_000354052.1
Coordinates 3255683..3255949 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD86_RS16725 (3251252) 3251252..3252985 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
EPD86_RS16730 (3252991) 3252991..3253701 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EPD86_RS16735 (3253726) 3253726..3254622 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
EPD86_RS16740 (3254734) 3254734..3255255 + 522 WP_001055867.1 flavodoxin FldB -
EPD86_RS16745 (3255295) 3255295..3255702 - 408 WP_000244767.1 protein YgfX Toxin
EPD86_RS16750 (3255683) 3255683..3255949 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
EPD86_RS16755 (3256192) 3256192..3257172 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EPD86_RS16760 (3257368) 3257368..3258027 - 660 WP_000250274.1 hemolysin III family protein -
EPD86_RS16765 (3258191) 3258191..3258501 - 311 Protein_3248 N(4)-acetylcytidine aminohydrolase -
EPD86_RS16770 (3258546) 3258546..3259979 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
EPD86_RS16775 (3260036) 3260036..3260779 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T116954 WP_000244767.1 NZ_CP034931:c3255702-3255295 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T116954 NZ_CP034931:c3255702-3255295 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT116954 WP_000354052.1 NZ_CP034931:c3255949-3255683 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT116954 NZ_CP034931:c3255949-3255683 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References