Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2364797..2365592 | Replicon | chromosome |
| Accession | NZ_CP034931 | ||
| Organism | Shigella flexneri strain 2013C-3749 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | Q93EZ7 |
| Locus tag | EPD86_RS12330 | Protein ID | WP_000854917.1 |
| Coordinates | 2364797..2365171 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D2ACA1 |
| Locus tag | EPD86_RS12335 | Protein ID | WP_001280958.1 |
| Coordinates | 2365218..2365592 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EPD86_RS12295 (2360406) | 2360406..2361344 | - | 939 | Protein_2384 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| EPD86_RS12305 (2361816) | 2361816..2362097 | - | 282 | Protein_2385 | DUF4942 domain-containing protein | - |
| EPD86_RS12310 (2362379) | 2362379..2363383 | - | 1005 | WP_001179707.1 | IS110-like element ISSfl8 family transposase | - |
| EPD86_RS12315 (2363455) | 2363455..2364015 | - | 561 | Protein_2387 | DUF4942 domain-containing protein | - |
| EPD86_RS12320 (2364100) | 2364100..2364297 | - | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| EPD86_RS12325 (2364309) | 2364309..2364800 | - | 492 | WP_000976828.1 | DUF5983 family protein | - |
| EPD86_RS12330 (2364797) | 2364797..2365171 | - | 375 | WP_000854917.1 | TA system toxin CbtA family protein | Toxin |
| EPD86_RS12335 (2365218) | 2365218..2365592 | - | 375 | WP_001280958.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| EPD86_RS12340 (2365755) | 2365755..2365976 | - | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| EPD86_RS12345 (2366039) | 2366039..2366515 | - | 477 | WP_005011624.1 | RadC family protein | - |
| EPD86_RS12350 (2366531) | 2366531..2367004 | - | 474 | WP_005011597.1 | antirestriction protein | - |
| EPD86_RS12355 (2367346) | 2367346..2368167 | - | 822 | WP_001234584.1 | DUF932 domain-containing protein | - |
| EPD86_RS12360 (2368267) | 2368267..2368440 | - | 174 | WP_000088744.1 | DUF905 family protein | - |
| EPD86_RS25775 (2368560) | 2368560..2368802 | - | 243 | WP_000348966.1 | DNA polymerase III subunit gamma/tau | - |
| EPD86_RS12375 (2369762) | 2369762..2370178 | - | 417 | WP_000275180.1 | AraC family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 2362379..2363383 | 1004 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14145.22 Da Isoelectric Point: 7.7761
>T116953 WP_000854917.1 NZ_CP034931:c2365171-2364797 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T116953 NZ_CP034931:c2365171-2364797 [Shigella flexneri]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13793.62 Da Isoelectric Point: 6.4651
>AT116953 WP_001280958.1 NZ_CP034931:c2365592-2365218 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT116953 NZ_CP034931:c2365592-2365218 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93EZ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K4PAV2 |