Detailed information of TA system TA116931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2331914..2332575
• Replicon: chromosome
• Accession: NZ_CP034909
• Organism: Ensifer alkalisoli strain YIC4027

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1E3VD76
• Locus tag: EKH55_RS11375
• Protein ID: WP_069458017.1
• Coordinates: 2331914..2332351 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EKH55_RS11380
• Protein ID: WP_106407814.1
• Coordinates: 2332348..2332575 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKH55_RS11335	2327586..2327867	+	282	WP_069458014.1	hypothetical protein	-
EKH55_RS29420	2328133..2328393	+	261	WP_069458015.1	hypothetical protein	-
EKH55_RS11350	2328874..2329080	+	207	WP_037471827.1	CsbD family protein	-
EKH55_RS11355	2329248..2329643	+	396	WP_069458016.1	PRC-barrel domain-containing protein	-
EKH55_RS11360	2329701..2330057	+	357	WP_037461821.1	hypothetical protein	-
EKH55_RS29425	2330122..2330271	+	150	WP_165614709.1	hypothetical protein	-
EKH55_RS11370	2331438..2331788	+	351	Protein_2205	Tn3 family transposase	-
EKH55_RS11375	2331914..2332351	-	438	WP_069458017.1	PIN domain-containing protein	Toxin
EKH55_RS11380	2332348..2332575	-	228	WP_106407814.1	CopG family transcriptional regulator	Antitoxin
EKH55_RS11385	2332679..2333635	-	957	WP_069458019.1	sigma-54-dependent Fis family transcriptional regulator	-
EKH55_RS11390	2333846..2335018	+	1173	WP_151611534.1	benzoate/H(+) symporter BenE family transporter	-
EKH55_RS11395	2335186..2335794	+	609	WP_151611535.1	50S ribosomal protein L25/general stress protein Ctc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15347.61 Da
• Isoelectric Point: 7.4783

>T116931 WP_069458017.1 NZ_CP034909:c2332351-2331914 [Ensifer alkalisoli]
VTFLLDVNVLIALIDPGHVAHDDAHHWFASTGRNAWATCPITENGVVRIVGNPKYPNFPGSPALVMEIVGKLRSLPGHCF
WPDDVSLVGSGNVASAKILTSAQVTDTYLLALAKVHGGQLATFDRKLSTAAVTQGNSALHLITAK

Nucleotide

• Length: 438 bp

>T116931 NZ_CP034909:c2332351-2331914 [Ensifer alkalisoli]
GTGACTTTCCTGCTCGACGTCAATGTATTGATTGCCTTGATCGATCCGGGCCACGTCGCCCATGATGATGCGCACCACTG
GTTCGCATCGACCGGCCGAAACGCGTGGGCCACCTGTCCTATTACCGAAAATGGCGTCGTCCGGATCGTCGGCAACCCGA
AATATCCCAATTTTCCCGGTTCGCCAGCACTTGTGATGGAAATCGTAGGGAAATTGCGATCGCTCCCCGGACATTGTTTC
TGGCCGGACGACGTGAGTCTTGTCGGTTCAGGCAACGTCGCTTCGGCAAAGATCCTGACTTCAGCCCAGGTGACGGACAC
CTACCTGCTTGCGCTCGCGAAGGTGCATGGCGGCCAGTTGGCGACTTTCGATCGCAAGCTATCAACCGCGGCCGTCACCC
AGGGCAATTCTGCCTTGCACCTCATCACCGCCAAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8127.31 Da
• Isoelectric Point: 7.0110

>AT116931 WP_106407814.1 NZ_CP034909:c2332575-2332348 [Ensifer alkalisoli]
MRTTLAIDDDVLVAAKAMAAQQHRSVGEVISDLARRSLRRPQSGGERNGIPLLSPRPDAPPVTLDMVNALRDELP

Nucleotide

• Length: 228 bp

>AT116931 NZ_CP034909:c2332575-2332348 [Ensifer alkalisoli]
ATGAGGACGACACTGGCGATCGACGACGATGTTCTGGTCGCCGCCAAGGCGATGGCGGCGCAACAGCACCGGAGCGTCGG
CGAAGTCATTTCCGACCTGGCGAGGCGTTCCCTCCGCCGGCCGCAGAGCGGCGGCGAGCGCAATGGAATTCCTCTGCTAT
CGCCCCGACCCGACGCGCCACCGGTGACACTCGATATGGTCAACGCATTGCGTGACGAATTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A1E3VD76