Detailed information of TA system TA116923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 509..1149
• Replicon: plasmid p4-AD3
• Accession: NZ_CP034906
• Organism: Roseomonas mucosa strain AD3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RMP42_RS22835
• Protein ID: WP_019463390.1
• Coordinates: 509..916 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RMP42_RS22840
• Protein ID: WP_019463391.1
• Coordinates: 913..1149 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMP42_RS22830	1..512	-	512	Protein_0	hypothetical protein	-
RMP42_RS22835 (RMP42_03206a)	509..916	-	408	WP_019463390.1	PIN domain-containing protein	Toxin
RMP42_RS22840 (RMP42_03207a)	913..1149	-	237	WP_019463391.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RMP42_RS22845 (RMP42_04622a)	1430..2560	+	1131	WP_115359424.1	helix-turn-helix domain-containing protein	-
RMP42_RS22850 (RMP42_04623)	2710..3753	+	1044	WP_051544367.1	hypothetical protein	-
RMP42_RS22855 (RMP42_04624)	3750..4058	+	309	WP_019463350.1	hypothetical protein	-
RMP42_RS22860 (RMP42_04625)	4078..4380	+	303	WP_265887128.1	hypothetical protein	-
RMP42_RS22865 (RMP42_02544a)	4429..5928	+	1500	WP_115359153.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35394	35394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-115)

Antitoxin

• SpoVT-AbrB domain (5-35)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14667.94 Da
• Isoelectric Point: 5.8528

>T116923 WP_019463390.1 NZ_CP034906:c916-509 [Roseomonas mucosa]
MNRPFLDTNILLYAALQPDPRSETARGLLARGGLVSVQVLNEFTAVARRKLRRPWPEVMQALAAIRTLCPAPRPLTVATH
EAAVGLAERLGYTIYDALILASALEAGCDTLFSEDLQDGQVVEGQLTIRNPFAAP

Nucleotide

• Length: 408 bp

>T116923 NZ_CP034906:c916-509 [Roseomonas mucosa]
ATGAACCGGCCCTTCCTCGATACCAACATCCTGCTCTACGCTGCCCTGCAGCCGGATCCGCGTTCCGAGACGGCGCGCGG
CCTCCTGGCCCGGGGCGGCCTGGTCAGCGTCCAGGTGCTCAACGAGTTCACCGCCGTCGCCCGCCGCAAGCTACGCCGGC
CCTGGCCCGAGGTCATGCAGGCGCTGGCCGCCATCCGGACTCTCTGTCCGGCGCCCCGGCCGCTCACCGTCGCGACGCAC
GAGGCGGCCGTTGGGCTGGCGGAGAGGCTGGGCTACACGATCTACGATGCCTTGATCCTGGCCTCGGCCCTGGAGGCCGG
CTGCGACACGCTCTTCTCCGAGGATCTGCAGGACGGGCAGGTGGTCGAAGGGCAACTGACCATCCGCAACCCCTTCGCCG
CGCCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8714.71 Da
• Isoelectric Point: 5.7066

>AT116923 WP_019463391.1 NZ_CP034906:c1149-913 [Roseomonas mucosa]
MQVSRWGNSLAVRLPAAVVEALGLREGDEVAIEVADSHTFRVARDRRRDEAIEALRGLGWHLPDGFRFSRDEANERGG

Nucleotide

• Length: 237 bp

>AT116923 NZ_CP034906:c1149-913 [Roseomonas mucosa]
ATGCAGGTCAGCCGCTGGGGCAACAGCCTCGCCGTTCGCCTCCCCGCCGCCGTGGTCGAGGCTTTGGGCCTGCGCGAGGG
CGACGAGGTCGCCATCGAGGTGGCCGACTCCCACACCTTCCGCGTGGCCCGCGACCGCCGCCGCGACGAGGCGATCGAGG
CGCTGCGCGGTCTCGGCTGGCACCTGCCCGACGGCTTCCGTTTCTCGCGCGACGAAGCCAACGAGCGTGGCGGATGA