Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) abiEi-abiEii/AbiEii-AbiEi
Location 2218106..2219525 Replicon chromosome
Accession NZ_CP034842
Organism Fusobacterium necrophorum subsp. necrophorum strain ATCC 25286

Toxin (Protein)


Gene name abiEi Uniprot ID A0A654RX45
Locus tag EO219_RS10250 Protein ID WP_074517856.1
Coordinates 2218106..2218939 (-) Length 278 a.a.

Antitoxin (Protein)


Gene name abiEii Uniprot ID A0A654S274
Locus tag EO219_RS10255 Protein ID WP_074517855.1
Coordinates 2218932..2219525 (-) Length 198 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO219_RS10220 2213411..2214157 - 747 WP_074517859.1 conjugal transfer protein -
EO219_RS10225 2214200..2214619 - 420 WP_200769404.1 hypothetical protein -
EO219_RS10230 2214773..2215750 - 978 WP_074517858.1 IS5 family transposase -
EO219_RS10235 2215842..2215991 - 150 WP_200769403.1 hypothetical protein -
EO219_RS10240 2215995..2217707 - 1713 WP_074517857.1 DNA topoisomerase 3 -
EO219_RS10245 2217875..2218060 - 186 WP_200769402.1 hypothetical protein -
EO219_RS10250 2218106..2218939 - 834 WP_074517856.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
EO219_RS10255 2218932..2219525 - 594 WP_074517855.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
EO219_RS10260 2219617..2221203 - 1587 WP_124019684.1 DUF4366 domain-containing protein -
EO219_RS10265 2221208..2221498 - 291 WP_074517853.1 conjugal transfer protein -
EO219_RS10270 2221510..2223807 - 2298 WP_200769401.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2201403..2239411 38008


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(33-266)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(6-50)


Sequences

Toxin        


Download         Length: 278 a.a.        Molecular weight: 32510.24 Da        Isoelectric Point: 6.1623

>T116919 WP_074517856.1 NZ_CP034842:c2218939-2218106 [Fusobacterium necrophorum subsp. necrophorum]
MNKDKLRAICQKLSKETGLSFNAVQTHYFLESILEKIASSDESENFIFKGGFLLANVIGIRQRNTVDIDFLIRRFSLTEE
NIKQRFEKILQSGNDNGITYEIQKIEEIRKEDEYGGFRITVLCRLENIRQIIPLDIATGDPITPSEISYEYKSIFSDKFF
EICAYNIETMLAEKIQTVYKRGAFNSRSKDFYDIYILYHLKKEEIDFEHLKEACKNTFKHRSTGFNIDSILDVLVDLKME
KDLKTRWSSYQKRFSYAGEINFDAVIDIMIELVKNIR

Download         Length: 834 bp

>T116919 NZ_CP034842:c2218939-2218106 [Fusobacterium necrophorum subsp. necrophorum]
ATGAATAAAGATAAGCTAAGAGCGATATGTCAAAAACTGTCGAAAGAAACAGGACTTAGCTTTAATGCTGTGCAAACACA
CTATTTTTTAGAAAGCATTTTAGAGAAAATAGCAAGTAGTGACGAGAGTGAAAACTTTATTTTTAAAGGTGGTTTTTTAC
TCGCAAATGTAATTGGAATAAGGCAGAGAAACACAGTAGATATTGATTTTTTGATAAGAAGATTTTCACTTACTGAAGAA
AACATCAAGCAAAGATTTGAAAAAATTCTTCAAAGCGGAAACGATAATGGAATTACTTATGAGATTCAAAAAATTGAAGA
AATCCGTAAAGAAGATGAATATGGAGGATTTAGAATTACAGTTCTTTGCAGACTTGAAAATATCAGGCAGATTATTCCAC
TCGATATAGCAACAGGCGATCCAATTACACCAAGTGAAATATCCTATGAATATAAAAGTATTTTTAGTGATAAGTTTTTT
GAAATTTGTGCATATAATATAGAAACAATGCTTGCTGAGAAGATTCAGACAGTATATAAAAGAGGGGCATTTAACAGCAG
AAGTAAGGATTTTTATGATATTTACATTCTATATCATTTGAAGAAAGAGGAAATAGACTTTGAGCATTTAAAGGAAGCTT
GTAAAAATACATTTAAGCATAGAAGCACAGGTTTTAATATAGATAGTATTCTTGATGTATTAGTCGACTTAAAAATGGAA
AAAGACCTCAAAACAAGGTGGAGTAGCTATCAAAAAAGATTTAGTTATGCAGGAGAAATAAATTTTGATGCTGTAATAGA
TATTATGATAGAATTAGTGAAAAACATAAGATAA

Antitoxin


Download         Length: 198 a.a.        Molecular weight: 23306.87 Da        Isoelectric Point: 8.5137

>AT116919 WP_074517855.1 NZ_CP034842:c2219525-2218932 [Fusobacterium necrophorum subsp. necrophorum]
MDYIKKIKKKLSKTGGVITSKELKNSNIPTIYLTRMVEKGELIRADRGIYIDSNGDYDEYYFFHNRCKVAVFSYVSALYL
QGFTDIIPNEMEVTVYKGYNTHRISGSVRIHYVTKEIYDLGMTECQTMFGNTVKVYDLERIICDFIKNRSEIETELLSKT
INKYVRYENKDLNKLYEYSKKMNIYEKVKEILEVVYE

Download         Length: 594 bp

>AT116919 NZ_CP034842:c2219525-2218932 [Fusobacterium necrophorum subsp. necrophorum]
ATGGATTATATAAAAAAGATAAAAAAAAAATTGAGTAAAACGGGTGGAGTTATAACAAGTAAGGAACTTAAAAACTCTAA
CATTCCTACAATATATCTCACACGAATGGTGGAAAAAGGTGAACTGATTCGTGCGGATAGAGGAATCTATATAGATTCAA
ACGGAGATTATGACGAGTATTATTTTTTTCATAATAGATGTAAGGTTGCTGTTTTTTCTTATGTATCCGCTCTTTATCTT
CAGGGGTTTACAGACATAATCCCGAATGAAATGGAAGTTACAGTATATAAAGGATACAATACTCATCGTATAAGTGGAAG
TGTAAGAATTCATTATGTAACAAAAGAAATTTATGATTTGGGTATGACAGAATGCCAAACAATGTTTGGGAATACAGTAA
AAGTTTATGACTTGGAGAGAATAATTTGCGATTTTATTAAAAATAGAAGTGAAATAGAAACAGAACTGCTTTCTAAAACG
ATTAACAAGTATGTCCGTTATGAAAATAAGGATTTGAATAAACTATATGAGTATTCTAAAAAAATGAATATATATGAAAA
AGTAAAAGAAATTTTGGAGGTTGTTTATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654RX45

Antitoxin

Source ID Structure
AlphaFold DB A0A654S274

References