Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/couple_hipB(antitoxin)
Location 274116..274662 Replicon plasmid pKM12v2
Accession NZ_CP034839
Organism Rahnella aquatilis strain KM25

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag EJP79_RS25040 Protein ID WP_205963719.1
Coordinates 274116..274361 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag EJP79_RS23775 Protein ID WP_121019978.1
Coordinates 274366..274662 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJP79_RS23750 269345..271444 + 2100 WP_119262374.1 lipase family protein -
EJP79_RS23755 271458..272198 + 741 WP_165497368.1 hypothetical protein -
EJP79_RS23760 272269..273009 + 741 WP_162906109.1 hypothetical protein -
EJP79_RS23765 273405..273821 + 417 WP_166861180.1 hypothetical protein -
EJP79_RS25035 273929..274183 - 255 WP_205963718.1 hypothetical protein -
EJP79_RS25040 274116..274361 - 246 WP_205963719.1 HipA N-terminal domain-containing protein Toxin
EJP79_RS23775 274366..274662 - 297 WP_121019978.1 helix-turn-helix transcriptional regulator Antitoxin
EJP79_RS23780 274850..275560 + 711 WP_013577982.1 GntR family transcriptional regulator -
EJP79_RS23785 275571..276770 - 1200 WP_013577983.1 2-dehydro-3-deoxy-6-phosphogalactonate aldolase -
EJP79_RS23790 277095..278495 + 1401 WP_014416613.1 PTS transporter subunit EIIC -
EJP79_RS23795 278502..279374 - 873 WP_112151483.1 fumarylacetoacetate hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaRAHN-2 mgtB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / hcp/tssD / hcp/tssD 1..556163 556163


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-74)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-68)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9422.50 Da        Isoelectric Point: 4.9670

>T116916 WP_205963719.1 NZ_CP034839:c274361-274116 [Rahnella aquatilis]
MRRAQQRLAIWMNGIPVGYWDKQKGEDRLQYSPEWTEDPQGRPLSLSLPFTPGNQVHRGNVVRDYFDNLLPDQQDPAQAL
D

Download         Length: 246 bp

>T116916 NZ_CP034839:c274361-274116 [Rahnella aquatilis]
ATGCGTCGTGCGCAGCAGCGTTTAGCGATATGGATGAACGGTATTCCTGTGGGGTACTGGGATAAGCAAAAAGGCGAGGA
TCGTTTACAGTACTCGCCCGAATGGACAGAGGACCCACAAGGGCGACCTTTGTCGCTTTCCCTGCCGTTTACGCCGGGTA
ATCAGGTGCATCGCGGCAATGTGGTTCGCGATTATTTTGATAATTTATTACCTGATCAGCAAGATCCTGCGCAGGCATTG
GATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11215.90 Da        Isoelectric Point: 9.5099

>AT116916 WP_121019978.1 NZ_CP034839:c274662-274366 [Rahnella aquatilis]
MNTVYPLKTLNQLRPLLVGFRKEKGLTQKDVSEKLGVTQQTYARLEANPATASLDRLFRVFNVLGIEMVFSSQSDPEKPS
MEEIKPDYLSTPARKEQW

Download         Length: 297 bp

>AT116916 NZ_CP034839:c274662-274366 [Rahnella aquatilis]
ATGAATACAGTCTATCCGTTAAAAACACTTAACCAGCTCCGTCCGTTACTGGTGGGGTTCCGTAAGGAAAAAGGTCTGAC
GCAAAAGGATGTGTCAGAAAAACTGGGCGTGACCCAGCAGACATATGCCCGACTGGAGGCAAACCCTGCTACTGCCAGCC
TTGACCGGCTGTTTCGGGTATTTAATGTGTTGGGCATTGAGATGGTGTTTTCCTCACAATCCGATCCTGAAAAACCCTCT
ATGGAGGAAATCAAGCCGGATTATTTAAGTACGCCCGCCAGAAAGGAACAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References